等离子体活化水在糠秕马拉色菌灭活中的应用

郝欣; 刘红霞; 柴守宁

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等离子体活化水在糠秕马拉色菌灭活中的应用

更新时间：2025-11-18

- 等离子体活化水在糠秕马拉色菌灭活中的应用
- The application of plasma-activated water for inactivating Malassezia furfur
- 默认刊物名称 2025年
- 作者机构：
  
  西安交通大学能源与动力工程学院环境科学与工程系,陕西省西安市710049
- 作者简介：
- 基金信息：
  
  等级孔分子筛封装高熵合金纳米团簇材料构建及其催化燃烧苯系物性能强化机制(52470128)
- DOI：
  中图分类号： Q89
- 收稿：2025-05-09，
  
  修回：2025-07-21，
  
  录用：2025-07-29，
- 稿件说明：
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郝欣, 刘红霞, 柴守宁. 等离子体活化水在糠秕马拉色菌灭活中的应用[J/OL]. 默认刊物名称, 2025.

HAO Xin, LIU Hongxia, CHAI Shouning. The application of plasma-activated water for inactivating Malassezia furfur[J/OL]. Moren Journal, 2025.
郝欣, 刘红霞, 柴守宁. 等离子体活化水在糠秕马拉色菌灭活中的应用[J/OL]. 默认刊物名称, 2025. DOI：

HAO Xin, LIU Hongxia, CHAI Shouning. The application of plasma-activated water for inactivating Malassezia furfur[J/OL]. Moren Journal, 2025. DOI：

摘要

针对现有灭活糠秕马拉色菌方法普适性较差，通常存在依从性低、不良反应及容易产生耐药性等问题，探索了等离子体活化水在糠秕马拉色菌灭活中的应用。通过低温等离子体技术制取不同类型的等离子体活化水，探讨物化性质的变化规律；根据菌落计数法分析糠秕马拉色菌菌体活性在灭活前后的变化规律；利用检测DNA及蛋白质泄漏量、胞外碱性磷酸酶（AKP）浓度以及紫外吸收物质含量等来分析灭活的作用机制。结果表明：在等离子体活化水中存在大量的活性物种，包括H

、H

、NO

等活性物种；当气体流量为5 L‧min

-1

和活化时间为25 min时，能够对糠秕马拉色菌造成明显的灭活效果，菌落数可降至0.98 lg（CFU‧mL

-1

）；导致菌体DNA和蛋白质泄漏、胞外AKP以及紫外吸收物质含量增加。研究可为等离子体活化水在防治糠秕马拉色菌中的应用提供理论支撑，有望成为微生物污染防治领域中绿色高效的应用方法。

Abstract

The investigation was motivated by the recognition of the limited applicability of existing methods for inactivating

Malassezia furfur

which often suffer from low compliance

adverse reactions

and the potential for drug resistance. The application of plasma-activated water in the inactivation of

Malassezia furfur

is explored. Different types of plasma-activated water are produced by low-temperature plasma technology to explore the change rule of physical and chemical properties

and the change rule of bacterial activity before and after inactivation is analyzed according to the colony counting method

DNA and protein leakage

extracellular alkaline phosphatase (AKP) concentration

and ultraviolet absorbing substance content are measured to analyze the mechanism of inactivation. The results indicate that there are a large number of active species present in plasma-activated water

including H

⁻

and other active species; When the gas flow rate is 5 L‧min

-1

and the activation time is 25 min

it can achieve a signi

ficant inactivation effect on

Malassezia furfur

reducing the colony count to 0.98 lg (CFU‧mL

-1

); This leads to increased leakage of bacterial DNA and proteins

extracellular AKP

and UV-absorbing substances. The study provides theoretical support for the application of plasma-activated water in the prevention and control of

Malassezia furfur

which is expected to become a green and efficient application method in the field of microbial pollution prevention and control.

关键词

Keywords

references

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