等离子体活化水在糠秕马拉色菌灭活中的应用

郝欣; 刘红霞; 柴守宁

doi:10.7652/xjtuxb202601008

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等离子体活化水在糠秕马拉色菌灭活中的应用

专题　“双碳”背景下的污染控制与资源化利用 | 更新时间：2026-01-15

- 等离子体活化水在糠秕马拉色菌灭活中的应用
- Application of Plasma-Activated Water in the Inactivation of Malassezia furfur
- 西安交通大学学报 2026年60卷第1期页码：72-80
- 作者机构：
  
  西安交通大学能源与动力工程学院，710049，西安
- 作者简介：
  
  作者简介：郝欣（1996—），女，博士生；
  刘红霞（通信作者），女，副教授，博士生导师。
- 基金信息：
  
  国家自然科学基金资助项目。(52470128)
- DOI：10.7652/xjtuxb202601008
  中图分类号： Q89
- 收稿：2025-05-09，
  
  纸质出版：2026-01-10
- 稿件说明：
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郝欣, 刘红霞, 柴守宁. 等离子体活化水在糠秕马拉色菌灭活中的应用[J]. 西安交通大学学报, 2026,60(1):72-80.

HAO Xin, LIU Hongxia, CHAI Shouning. Application of Plasma-Activated Water in the Inactivation of Malassezia furfur[J]. Journal of Xi'an Jiaotong University, 2026, 60(1): 72-80. DOI: 10.7652/xjtuxb202601008.
郝欣, 刘红霞, 柴守宁. 等离子体活化水在糠秕马拉色菌灭活中的应用[J]. 西安交通大学学报, 2026,60(1):72-80. DOI： 10.7652/xjtuxb202601008.

HAO Xin, LIU Hongxia, CHAI Shouning. Application of Plasma-Activated Water in the Inactivation of Malassezia furfur[J]. Journal of Xi'an Jiaotong University, 2026, 60(1): 72-80. DOI: 10.7652/xjtuxb202601008. DOI：

摘要

针对现有灭活糠秕马拉色菌方法普适性较差、依从性低、不良反应及容易产生耐药性等问题，探索了等离子体活化水在糠秕马拉色菌灭活中的应用。通过低温等离子体技术制取不同类型的等离子体活化水，探讨物化性质的变化规律；根据菌落计数法分析糠秕马拉色菌菌体活性在灭活前后的变化规律；利用检测DNA及蛋白质泄漏量、胞外碱性磷酸酶（AKP）活性以及紫外吸收物质含量等来分析灭活的作用机制。结果表明：在等离子体活化水中存在大量活性物种，包括H

、H

、

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366803&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366803&type=small

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366803&type=middle

、

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366804&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366804&type=small

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366804&type=middle

等；当气体流量为5 L·min

-1

、活化时间为25 min时，等离子体活化水能够对糠秕马拉色菌造成明显的灭活效果，菌落数可降至10

0.98

CFU·mL

-1

；等离子体活化水可导致菌体DNA和蛋白质泄漏，胞外AKP以及紫外吸收物质含量增加。研究可为等离子体活化水在防治糠秕马拉色菌中的应用提供理论支撑。

Abstract

To address the limitations of existing methods for inactivating

Malassezia furfur

，such as poor universality，low compliance，adverse reactions，and tendency to induce drug resistance，the application of plasma-activated water (PAW)for its inactivation is explored.Different types of PAW are prepared using low-temperature plasma technology，and the variations in their physicochemical properties are investigated.Changes in the viability of

Malassezia furfur

before and after inactivation are analyzed based on the colony counting method.The inactivation mechanism is analyzed by detecting DNA and protein leakage，extracellular alkaline phosphatase (AKP)activity，and the content of UV-absorbing substances.The results indicate that PAW contains a large number of reactive species，includingH

，H

，

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366805&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366805&type=small

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366805&type=middle

，

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366791&type=

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366791&type=small

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=112366791&type=middle

，etc.Significant inactivation of

Malassezia furfur

is achieved when the gas flow rate is 5 L·min

-1

and the activation time is 25 min，reducing the colony count to 10

0.98

CFU·mL

-1

.The inactivation leads to increased leakage of DNA and proteins，as well as elevated levels of extracellular AKP and UV-absorbing substances.This study provides theoretical support for the application of PAW in preventing and controlling

Malassezia furfur

关键词

Keywords

references

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