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CO2体积分数调控胞外有机物特性诱导小球藻自絮凝的实验研究
—————————————————————— | 更新时间：2025-09-11
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CO2体积分数调控胞外有机物特性诱导小球藻自絮凝的实验研究
Experimental Study on CO2 Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of
Chlorella vulgaris - 西安交通大学学报 2025年59卷第9期 页码：122-131
- 作者机构：
西安交通大学动力工程多相流国家重点实验室， 710049，西安
- 作者简介：
魏璇（1996—），女，博士生
郭烈锦，男，教授，博士生导师，中国科学院院士。
- 基金信息：国家自然科学基金资助项目(52488201)
DOI：10.7652/xjtuxb202509012
中图分类号： TK6收稿：2025-01-06，
网络首发：2025-04-15，
纸质出版：2025-09-10
- 稿件说明：
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魏璇, 于归源, 郭翔, 等. CO2体积分数调控胞外有机物特性诱导小球藻自絮凝的实验研究[J]. 西安交通大学学报, 2025,59(9):122-131. DOI: 10.7652/xjtuxb202509012.
WEI Xuan, YU Guiyuan, GUO Xiang, et al. Experimental Study on CO2 Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of
Chlorella vulgaris [J]. Journal of Xi’an Jiaotong University, 2025, 59(9): 122-131. DOI: 10.7652/xjtuxb202509012.魏璇, 于归源, 郭翔, 等. CO2体积分数调控胞外有机物特性诱导小球藻自絮凝的实验研究[J]. 西安交通大学学报, 2025,59(9):122-131. DOI: 10.7652/xjtuxb202509012. DOI：
WEI Xuan, YU Guiyuan, GUO Xiang, et al. Experimental Study on CO2 Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of
Chlorella vulgaris [J]. Journal of Xi’an Jiaotong University, 2025, 59(9): 122-131. DOI: 10.7652/xjtuxb202509012. DOI：
CO2体积分数调控胞外有机物特性诱导小球藻自絮凝的实验研究
摘要
针对微藻收获过程中化学絮凝剂成本高、环境风险大以及自絮凝效率较低的问题，系统研究了通过调控CO
2
体积分数诱导小球藻自絮凝的作用机制，重点探讨胞外有机物（EOM）在该过程中的关键调节作用。实验采用3种CO
2
体积分数（约0.04%、15%、25%）培养条件，通过分析EOM特性、pH和无机盐质量浓度等参数，综合评估了不同培养条件下小球藻的自絮凝行为及其影响因素。实验结果表明，CO
2
体积分数显著影响EOM的分泌量，进而改变小球藻的自絮凝效率。相较于CO
2
体积分数为15%和25%的培养组，CO
2
体积分数约为0.04%时培养的小球藻单位生物量分泌的蛋白质和多糖的质量浓度分别提高至其4~7倍和3~5倍，自絮凝效率最高达86%。去除EOM后，小球藻在12 h后的自絮凝效率下降了13%~24%，进一步验证了EOM在细胞自絮凝过程中的关键调控作用。相比之下，pH和无机盐质量浓度变化对自絮凝效率影响不显著。从CO
2
供给角度出发，系统揭示了CO
2
、EOM、自絮凝三者之间的关联机制，为低成本、高效率微藻的液固分离工艺的优化提供了实验参考。
Abstract
To address the challenges of high chemical flocculant costs
high environmental risks
and low self-flocculation efficiency in microalgae harvesting
this study systematically investigates the mechanism of inducing self-flocculation of
Chlorella vulgaris
by regulating the CO
2
concentration
with a focus on the key regulatory role of extracellular organic matter (EOM) in the process. Three CO
2
concentration cultivation conditions (about 0.04%
15%
and 25%) are employed
and parameters such as EOM characteristics
pH and concentrations of inorganic salts are analyzed to comprehensively evaluate the self-flocculation behavior of
Chlorella vulgaris
and its influencing factors under different cultivation conditions. The experimental results indicate that the CO
2
conce
ntration significantly affects the secretion of EOM
thus altering the self-flocculation efficiency of
Chlorella vulgaris
. Compared to the 15% and 25% CO
2
concentration cultivation groups
the protein and polysaccharide concentrations secreted per unit biomass of
Chlorella vulgaris
cultivated at approximately 0.04% CO
2
concentration increase by 4 to 7 times and 3 to 5 times
respectively
with the highest self-flocculation efficiency reaching 86%. After the removal of EOM
the self-flocculation efficiency of
Chlorella vulgaris
decreases by 13% to 24% after 12 hours
further validating the critical regulatory role of EOM in the self-flocculation process. In contrast
variations in pH and inorganic salt concentrations have a negligible effect on self-flocculation efficiency. From the perspective of CO
2
supply
this study reveals the interconnection mechanism among CO
2
EOM
and self-flocculation
providing experimental references for optimizing low-cost
high-efficiency liquid-solid separation processes for microalgae.
关键词
Keywords
references
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