酶交联明胶水凝胶性能研究及仿生微流道制备

陈若梦; 王宏; 黄亚江; 刘亚雄; 贺健康; 李涤尘

doi:10.7652/xjtuxb201712021

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酶交联明胶水凝胶性能研究及仿生微流道制备

更新时间：2025-07-09

- 酶交联明胶水凝胶性能研究及仿生微流道制备
- Properties of Enzymatically Crosslinked Gelatin and Fabrication of Bio-Microchannel
- 西安交通大学学报 2017年51卷第12期页码：143-149
- 作者机构：
  
  西安交通大学机械制造系统工程国家重点实验室,西安,710049
- 作者简介：
- 基金信息：
  
  : 2017-03-14。作者简介: 陈若梦(1987—),女,博士生;刘亚雄(通信作者),男,教授,博士生导师。基金项目: 国家自然科学基金资助项目(51675416);陕西省统筹项目(2015KTTSGY-04)
- DOI：10.7652/xjtuxb201712021
  中图分类号： Q811.2
- 网络首发：2017-12-10，
  
  纸质出版：2017
- 稿件说明：
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陈若梦, 王宏, 黄亚江, 等. 酶交联明胶水凝胶性能研究及仿生微流道制备[J]. 西安交通大学学报, 2017,51(12):143-149.

Properties of Enzymatically Crosslinked Gelatin and Fabrication of Bio-Microchannel[J]. 2017, 51(12): 143-149.
陈若梦, 王宏, 黄亚江, 等. 酶交联明胶水凝胶性能研究及仿生微流道制备[J]. 西安交通大学学报, 2017,51(12):143-149. DOI： 10.7652/xjtuxb201712021.

Properties of Enzymatically Crosslinked Gelatin and Fabrication of Bio-Microchannel[J]. 2017, 51(12): 143-149. DOI： 10.7652/xjtuxb201712021.

摘要

针对当前水凝胶构建实质性功能器官存在制备血管网络困难的问题

采用了转谷氨酰胺酶(mTG酶)交联明胶构建明胶水凝胶圆形仿生微流道的方法。研究了明胶水凝胶的力学性能、成形性能及生物性能与明胶浓度的关系

提出了明胶水凝胶构建微流道结构的工艺条件

评价了微流道构建工艺的成型精度、力学性能和内皮化功能。结果表明:随着水凝胶浓度的提高

水凝胶的力学性能成线性增加

微结构的成型性能也逐步改善

但是浓度过高生物性能反而降低

明胶质量浓度为0.1 g/mL的水凝胶综合性能相对较好

可用于构建圆形仿生微流道; 采用的微流道成型工艺保持了原有水凝胶的力学性能

但是存在一定的成型误差

细胞在微流道内分布均匀

实现了微流道的内皮化。通过研究不同浓度的酶交联明胶水凝胶

制备的仿生微流道有望用于实质性功能器官的仿生再建。

Abstract

A new strategy is adopted to construct the hydrogel microchannel with the enzymatically-crosslinked gelatin by evaluating the different concentration gelatin hydrogel

which aims to solve the difficulty of fabricating circular microchannel by soft hydrogel. The relationship of the gelatin properties(mechanical

forming and biological)with its concentration is revealed and discussed

and the molding process of gelatin hydrogel microchannel is proposed. The structural

mechanical and endothelialization properties of gelatin hydrogel microchannel are also studied. The experimental results indicate that with the increasing gelatin concentration

the mechanical property and microstructure replication property of the composite hydrogels are gradually improved

however

superior concentration causes lower biological performance. Hence

the hydrogel with 0. 1 g/mL gelatin is chosen as the optimal composition to build circular microchannel. The approach for fabricating microchannel retains the original mechanical properties of the hydrogel

although certain error exists in the forming process from characterizing morphology and the dimension of the microchannel. Besides

the microchannel gelatin facilitates attaching and spreading of human umbilical endothelial cells(HUVECs)to form a uniform endothelialized layer around the channel surface. Following the evaluation of different concentrations of enzymatically crosslinked gelatin hydrogel

the biomimetic microchannel can be expected to apply to reconstruction of substantive functional organs.

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references

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