软组织支架3D打印/压印成形工艺研究

王振; 王亚宁; 陈冬; 郑兆波; 刘亚雄; 贺健康; 王玲; 李涤尘; 靳忠民

doi:10.7652/xjtuxb201508023

您当前的位置：

首页 >

文章列表页 >

软组织支架3D打印/压印成形工艺研究

更新时间：2025-07-09

- 软组织支架3D打印/压印成形工艺研究
- 3D Printing/Imprinting Molding Process of Soft Tissue Scaffolds
- 西安交通大学学报 2015年49卷第8期页码：141-146
- 作者机构：
  
  西安交通大学机械制造系统工程国家重点实验室,西安,710049
- 作者简介：
- 基金信息：
  
  : 2015-01-31。作者简介: 王振(1990—),男,硕士生;刘亚雄(通信作者),男,教授,博士生导师。基金项目: 国家自然科学基金资助项目(51275387);中央高校基本科研业务费专项资金资助项目
- DOI：10.7652/xjtuxb201508023
  中图分类号： Q811.2
- 网络首发：2015-08-10，
  
  纸质出版：2015
- 稿件说明：
移动端阅览
王振, 王亚宁, 陈冬, 等. 软组织支架3D打印/压印成形工艺研究[J]. 西安交通大学学报, 2015,49(8):141-146.

3D Printing/Imprinting Molding Process of Soft Tissue Scaffolds[J]. 2015, 49(8): 141-146.
王振, 王亚宁, 陈冬, 等. 软组织支架3D打印/压印成形工艺研究[J]. 西安交通大学学报, 2015,49(8):141-146. DOI： 10.7652/xjtuxb201508023.

3D Printing/Imprinting Molding Process of Soft Tissue Scaffolds[J]. 2015, 49(8): 141-146. DOI： 10.7652/xjtuxb201508023.

摘要

针对当前采用3D打印技术制作软组织工程支架效率低、对细胞损伤大、圆形流道成形精度低等问题

提出了压印与打印技术相结合的直接成形新方法。首先采用压印方法结合光交联固化方法来制造具有流道结构的单层软组织支架

之后利用打印方法沿着流道注入明胶等温敏材料

通过低温环境使流道内的明胶等温敏材料固化起支撑作用

并逐层累积形成立体结构

最后利用温度控制使流道内的明胶融化

形成管道

最终形成具有流道结构的软组织支架。针对该工艺

研制开发出了相应自动化成形设备

结果表明

当扫描间距为51 mm时

光强均匀度最高

用该工艺制作出来的支架

圆弧形流道结构清晰平滑

流道相通性很好。研究结果有利于软组织工程支架微结构的精确制造

可望进一步用于细胞打印并提高细胞成活率和成形效率。

Abstract

Focusing on the low efficiency

lager cell damage and low forming accuracy of circular channel in soft tissue scaffolds construction by 3D printing technology

a novel method is presented to fabricate scaffolds by combining imprinting with 3D printing techniques. The imprinting technique is combined with light crosslinking method to make single-layer soft tissue scaffold with flow channel structure. Then

gelatin

a kind of temperature-sensitive material

is injected along the flow channel by printing technique to cure in the low temperature environment to support the upper layers. A stereoscopic structure is formed via the layer-by-layer accumulation. The gelatin is finally melted in the flow channel by temperature control to form pipelines. An automatic fabrication device is developed to realize the strategy. The results demonstrate that the light uniformity reaches the maximum when the scanning interval reaches 51 mm

and that the arc-type flow channel structure is clearly visible and interlinked. The approach is beneficial to accurate manufacture of soft tissue scaffolds

and it can be expected to improve cell survival rate and forming efficiency in cell printing.

关键词

Keywords

references

ATALA A, BAUER S B, SOKER S, et al. Tissue-engineered autologous bladders for patients needing cystoplasty [J]. The Lancet, 2006, 367(9518): 1241-1246.

GROEBER F, HOLEITER M, HAMPEL M, et al. Skin tissue engineering: in vivo and in vitro applications [J]. Advanced Drug Delivery Reviews, 2011, 63(4/5): 352-366.

王烨, 贺健康, 刘亚雄, 等. 三维微流道支架直接成形压印方法 [J]. 西安交通大学学报, 2012, 46(10): 116-120.

WANG Ye, HE Jiankang, LIU Yaxiong, et al. Direct imprinting of three-dimensional microfluidic scaffolds [J]. Journal of Xi'an Jiaotong University, 2012, 46(10): 116-120.

MIRONOV V, KASYANOV V, MARKWALD R R. Organ printing: from bioprinter to organ biofabrication line [J]. Curr Opin Biotechnol, 2011, 22: 667-673.

YAN Y, WANG X, PAN Y, et al. Fabrication of viable tissue-engineered constructs with 3D cell-assembly technique [J]. Biomaterials, 2005, 26(29): 5864-5871.

LIU T V, CHEN A A, CHO L M, et al. Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels [J]. Faseb J, 2007, 21(3): 790-801.

LIN H, ZHANG D, ALEXANDER P G, et al. Application of visible light-based projection stereolithography for live cell-scaffold fabrication with designed architecture [J]. Biomaterials, 2013, 34(2): 331-339.

朱林重, 连岑, 靳忠民, 等. PEGDA水凝胶的光固化成形工艺及其性能评价 [J]. 西安交通大学学报, 2012, 46(10): 121-126.

ZHU Linzhong, LIAN Qin, JIN Zhongmin, et al. Fabrication and evaluation of PEGDA hydrogel using stereo-lithography for cartilage tissue engineering application [J]. Journal of Xi'an Jiaotong University, 2012, 46(10): 121-126.

HE J K, LI P C, LIU Y X, et al. Preparation of chito san-gelatin hybrid scaffolds with well organized microstructures for hepatic tissue engineering [J]. Acta Biomaterialia, 2009, 5(1): 453-461.

DADO D, LEVENBERG S. Cell scaffold mechanical interplay within engineered tissue [J]. Seminars in Cell Developmental Biology, 2009, 20(6): 656-664.

SONG Y S, LIN R L, MONTESANO G, et al. Engineered 3D tissue models for cell-laden microfluidic channels [J]. Analytical and Bioanalytical Chemistry, 2009, 395(1): 185-193.

MAO M, HE J K, LIU Y X, et al. Ice-template-induced silk fibroin-chitosan scaffolds with predefined microfluidic channels and fully porous structures [J]. Acta Biomaterialia, 2011, 8(6): 2175-2184.

罗楠,王泉,刘红霞.一种快速3D打印分层方向确定算法.2015,49(5):140-146.[doi:10.7652/xjtuxb201505022]

张文友,贺健康,刘亚雄,等.韧带-骨支架仿生界面设计与制造方法.2014,48(10):137-142.[doi:10.7652/xjtuxb201410 022]

岳桂华,滕奇志,何小海,等.岩心三维图像修复算法.2014,48(9):37-42.[doi:10.7652/xjtuxb201409007]

张琳彦,朱利.以多幅图像非几何约束线段匹配重建建筑物外立面三维线段模型.2014,48(4):15-19+25.[doi:10.7652/xjtuxb201404003]

朱琳,贺健康,刘亚雄,等.琼脂糖/胶原复合水凝胶的制备及性能研究.2013,47(10):121-126.[doi:10.7652/xjtuxb2013 10021]

李世泽,魏正英,杜军,等.微压印中抗蚀剂填充行为研究.2013,47(9):126-133.[doi:10.7652/xjtuxb201309021]

王烨,贺健康,刘亚雄,等.三维微流道支架直接压印成形方法.2012,46(10):116-120.[doi:10.7652/xjtuxb201210020]

朱林重,连芩,靳忠民,等.PEGDA水凝胶的光固化成形工艺及其性能评价.2012,46(10):121-126.[doi:10.7652/xjtuxb201210021]

郝星,贺健康,高琨,等.蚕丝蛋白/明胶多孔肝组织支架的制备及性能研究.2011,45(11):121-126.[doi:10.7652/xjtuxb 201111023]

赵倩,刘亚雄,高琨,等.肝组织工程支架的仿生设计与有限元分析.2011,45(8):108-112.[doi:10.7652/xjtuxb201108 019]

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

三维微流道支架直接压印成形方法

超临界水异常区域热力学特性研究

扇贝仿生密封腔室内超临界二氧化碳泄漏流湍动能耗散机理研究

隧道内燃料电池汽车氢气泄漏扩散特性及影响因素

混合电解槽阵列协同的风光耦合制氢系统双层多目标优化