采用抗体包被纳米金多层自组装膜的HCG安培免疫传感器

干宁 1; 倪敏君 2; 郑磊 3; 王峰 1; 李天华 1

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采用抗体包被纳米金多层自组装膜的HCG安培免疫传感器

更新时间：2025-07-09

- 采用抗体包被纳米金多层自组装膜的HCG安培免疫传感器
- Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode
- 西安交通大学学报 2010年44卷第7期页码：114-119
- 作者机构：
  
  新型功能材料及其制备科学国家重点实验室, 宁波大学材料科学与化学工程学院,浙江,宁波,315211
- 作者简介：
- 基金信息：
  
  : 2009-12-15. 作者简介: 干宁(1974-),男,副教授,博士. 基金项目: 国家自然科学基金资助项目(20805024);广东省科技计划资助项目(2008A050200006, 2008C50012, 2009D10010)
- DOI：
  中图分类号： O657.1
- 网络首发：2010-07-10，
  
  纸质出版：2010
- 稿件说明：
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干宁 1, 倪敏君 2, 郑磊 3, 等. 采用抗体包被纳米金多层自组装膜的HCG安培免疫传感器[J]. 西安交通大学学报, 2010,44(7):114-119.

Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode[J]. 2010, 44(7): 114-119.
干宁 1, 倪敏君 2, 郑磊 3, 等. 采用抗体包被纳米金多层自组装膜的HCG安培免疫传感器[J]. 西安交通大学学报, 2010,44(7):114-119. DOI：

Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode[J]. 2010, 44(7): 114-119. DOI：

摘要

研制了一种采用人绒毛膜促性腺激素抗体(anti-HCG)包被巯基丁二酰胺(HL)/纳米金(AuNPs)多层膜修饰金电极的免疫传感器(Au|{HL/AuNPs}

-anti-HCG)

用于检测人唾液中的HCG.通过层层自组装技术在Au电极表面交替修饰多层{HL/AuNPs}

膜

再通过膜内的AuNPs吸附anti-HCG

构筑了HCG安培免疫传感器.以K

Fe(CN)

为探针

在pH 7.0的PBS缓冲液中加入HCG

随着HCG与电极表面anti-HCG发生特异性免疫反应

形成的抗原/抗体免疫复合物使得K

Fe(CN)

在电极表面的响应电流减小

减小量(I

)与膜表面的免疫反应程度相关

以此作为HCG定量的依据.对各修饰电极表面进行了表征和定量分析.实验表明:该免疫传感电极中{HL/AuNPs}

膜内存在的大量AuNPs显著增加了anti-HCG吸附量

从而使得其HCG检测范围变宽(0.1～100 mg/L)

检出下限为0.056 mg/L(3σ).

Abstract

An amperometric immunosensor for human chorionic gonadotrophin(HCG)in the saliva is developed based on HCG antibody(anti-HCG)coated mercapto succinic amide(HL)/gold nanoparticles(AuNPs)multilayer membrane modified Au electrode. HL/AuNPs monolayers are modified alternatively and form multiple membranes through Au-NH

and Au-S bonds by layer-by-layer self-assembly technology. K

Fe(CN)

is employed as electron probe for detection. The antigen-antibody immune complex formed on the electrode surface is able to hinder electron transport

so the redox current of K

Fe(CN)

is decreased. The decreased value of ΔI

is related to the extent of immune-reaction on membrane surface

which serves as the quantitative basis of HCG content. The experimental results show that multilayer AuNPs membrane can adsorb more anti-HCG

and obviously broaden the linear detection range of HCG to 0.1-100 mg/L with detection limit of 0.056 mg/L(3σ).

关键词

Keywords

references

CHAI Rong, YUAN Ruo, CHAI Yaqin, et al. Amperometric immunosensors based on layer-by-layer assembly of gold nanoparticles and methylene blue on thiourea modified glassy carbon electrode for determination of human chorionic gonadotrophin [J]. Talanta, 2008,74(5):1330-1336.

CHIAPPIN S, ANTONELLI G, GATTI R, et al. Saliva specimen: a new laboratory tool for diagnostic and basic investigation [J]. Clinica Chimica Acta, 2007,383(1/2):30-40.

CHEN Jin, YAN Feng, DAI Zong, et al. Reagentless amperometric immunosensor for human chorionic gonadotrophin based on direct electrochemistry of horseradish peroxidase [J]. Biosens, Bioelectron, 2005,21(2):330-336.

干宁,李榕生,陈亚东,等. 基于抗体包被金磁纳米微粒修饰电极的HIVp24和gp36安培联检芯片[J].西安交通大学学报,2009,43(7):115-119.

GAN Ning, LI Rongsheng, CHEN Yadong, et al. Micro fluidic amperometric immunosensor chip for HIV-p24 and gp36 antigens based on gold magnetic nanoensemble electrode [J]. Journal of Xi'an Jiaotong University, 2009,43(7):115-119.

CHEN Jin, TANG Jinhai, YAN Feng, et al. A gold nanoparticles/sol-gel composite architecture for encapsulation of immunoconjugate for reagentless electrochemical immunoassay [J]. Biomaterials, 2006,27(10):2313-2321.

CHEN Jin, YAN Feng, TANG Jinhai, et al. Gold nanoparticles doped three-dimensional sol-gel matrix for amperometric human chorionic gonadotrophin immunosensor [J]. Electroanalysis, 2006,18(17):1696-1702.

GUO Wei, HU Naifei. Interaction of myoglobin with poly(methacrylic acid)at different pH in their layer-by-layer assembly films: an electrochemical study [J]. Biophysical Chemistry, 2007,129(2/3):163-171.

IDEGAMI K, CHIKAE M, KERMAN K, et al. Gold nanoparticle-based redox signal enhancement for sensitive detection of human chorionic gonadotropin hormone [J]. Electroanalysis, 2008,20(1):14-21.

WARAKORN L, PROESPICHAYA K, BO M, et al. A reusable capacitive immunosensor for human chorionic gonadotrophin detection using thiourea modified gold electrode [J]. Analytica Chimica Acta, 2006,561(1/2):55-61.

YANG Gongmei, CHANG Yanbin, YANG Hui, et al. The preparation of reagentless electrochemical immunosensor based on a nano-gold and chitosan hybrid film for human chorionic gonadotrophin [J]. Analytica Chimica Acta, 2009,644(1/2):72-77.

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