PEGDA水凝胶的光固化成形工艺及其性能评价

朱林重 1; 连芩 1; 靳忠民 1; 2; 张维杰 1; 李涤尘 1

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PEGDA水凝胶的光固化成形工艺及其性能评价

更新时间：2025-07-09

- PEGDA水凝胶的光固化成形工艺及其性能评价
- Fabrication and Evaluation of PEGDA Hydrogel by Stereo-Lithography for Cartilage Tissue Engineering
- 西安交通大学学报 2012年46卷第10期页码：121-126
- 作者机构：
  
  西安交通大学机械制造系统工程国家重点实验室,西安,710054
- 作者简介：
- 基金信息：
  
  : 2012-03-07. 作者简介: 朱林重(1987-),男,硕士生;连芩(通信作者),女,副教授. 基金项目: 国家自然科学基金资助项目(50775178, 51075320);西安市创新支撑计划资助项目(CXY1014(2));中央高校基本科研业务费专项资金资助项目
- DOI：
  中图分类号： TH16;TB17
- 网络首发：2012-10-10，
  
  纸质出版：2012
- 稿件说明：
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朱林重 1, 连芩 1, 靳忠民 1, 等. PEGDA水凝胶的光固化成形工艺及其性能评价[J]. 西安交通大学学报, 2012,46(10):121-126.

Fabrication and Evaluation of PEGDA Hydrogel by Stereo-Lithography for Cartilage Tissue Engineering[J]. 2012, 46(10): 121-126.
朱林重 1, 连芩 1, 靳忠民 1, 等. PEGDA水凝胶的光固化成形工艺及其性能评价[J]. 西安交通大学学报, 2012,46(10):121-126. DOI：

Fabrication and Evaluation of PEGDA Hydrogel by Stereo-Lithography for Cartilage Tissue Engineering[J]. 2012, 46(10): 121-126. DOI：

摘要

采用光固化成形技术制备三维结构软骨组织工程聚乙二醇双丙烯酸酯(PEGDA)水凝胶

并对其成形工艺参数进行对比研究.对聚乙二醇(400)双丙烯酸酯(PEG(400)DA)溶液光固化成形单条线及单层面的形貌尺寸进行测量

研究PEG(400)DA体积分数、激光扫描速度以及扫描线间距等参数对测量结果影响.对制备的PEGDA水凝胶成形尺寸、成分、吸水率、压缩弹性模量等进行评价

结果表明:随着曝光量以及φ(PEG(400)DA)的增加

水凝胶单条线的固化厚度与固化宽度均增加; 随着激光扫描填充向量间距的增加

水凝胶单层面的固化厚度减小; 随着φ(PEG(400)DA)的增加

固化后的水凝胶吸水率降低

压缩弹性模量增大.采用φ(PEG(400)DA)为30%的溶液

激光器功率为0.1 W

扫描速度为1 800 mm/s

填充向量间距为0.15 mm

能够制备出定制化膝关节水凝胶软骨支架

其吸水率为191.67%

平均压缩弹性模量为0.75 MPa

满足软骨组织工程的需求.

Abstract

The stereo-lithography technique for fabricating biocompatible polyethyleneglycol diacrylate(PEGDA)hydrogel with a controlled accurate architecture is developed for cartilage tissue engineering application

and the different laser scanning parameters for single-line hydrogel or 2-D layer hydrogel are comparatively investigated

The cure parameters of the single-line and 2-D layer of hydrogel scaffold are affected by PEG(400)DA content of the solution

the laser scanning speed and hatch space. Hydrogel scaffold properties

such as structure accuracy

composition

and water absorption and compression modulus are experimentally confirmed. The results showed that: Cure depth and width increase positively with the energy dosage of laser and the PEG(400)DA content of solution; Cure depth scales inversely with the hatch space; Water absorption decreases and the compression modulus increases as the PEG(400)DA content of solution varies from 30% to 60%. The optimal laser process parameters include: 0.1 W laser power

1 800 mm/s laser scanning speed

0.15 mm hatch space and 30% content of φ(PEG(400)DA)solution

customized hydrogel scaffold of knee cartilage was obtained with 191.67% water absorption rate and 0.75 MPa average compression modulus. The stereo-lithographic PEGDA hydrogel technique perfectly meets the requirements for cartilage tissue engineering.

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references

GEEVER L M, MíNGUEZ C M, DEVINE D M, et al. The synthesis, swelling behavior and rheological properties of chemically crosslinked thermosensitive copolymers based on N-isopropylacrylamide [J]. Journal of Materials Science, 2007, 42(12): 4136-4148.

DEVINE D M, HIGGINBOTHAM C L. The synthesis of a physically crosslinked NVP based hydrogel [J]. Polymer, 2003, 44(26): 7851-7860.

PEPPAS N A, BURES P, LEOBANDUNG W, et al. Hydrogels in pharmaceutical formulations[J]. Eur J Pharm Biopharm, 2000, 50(1):27-46.

RAVICHANDRAN P, SHANTHA K L, RAO K P. Preparation, swelling characteristics and evaluation of hydrogels for stomach specific drug delivery[J]. Int J Pharm, 1997, 154(1):89-94.

SUN Shaohua, CAO Hui, SU Haijia, et al. Preparation and characterization of a novel injectable in situ cross-linked hydrogel [J]. Polymer Bulletin, 2009, 62(5):699-711.

ARCAUTE K, MANN B, WICKER R. Stereolithography of spatially controlled multi-material bioactive poly(ethylene glycol)scaffolds [J]. Acta Biomaterialia, 2010, 6(3): 1047-1054.

ARCAUTE K, MANN B K, WICKER R B. Stereolithography of three-dimensional bioactive poly(ethylene glycol)constructs with encapsulated cells [J]. Annals of Biomedical Engineering, 2006, 34(9): 1429-1441.

DHARIWALA B, HUNT E, BOLAND T. Rapid prototyping of tissue-engineering constructs, using photopolymerizable hydrogels and stereolithography[J]. Tissue Engineering, 2004, 10(9/10): 1316-1322.

KIM G H, SON J G. 3D polycarprolactone(pcl)scaffold with hierarchical structure fabricated by a piezoelectric transducer(pzt)-assisted bioplotter [J]. Appl Phys: A Materials Science Processing, 2009, 94:781-785.

BOLAND T, TAO X, DAMON B J, et al. Drop-on-demand printing of cells and materials for designer tissue constructs [J]. Materials Science Engineering: C Biomimetic and Superamolecular Systems 2007, 27:372-376

LANDERS R, MULHAUPT R. Desktop manufacturing of complex objects, proto-types and biomedical scaffolds by means of computer-assisted design combined with computer-guided 3D plotting of polymers and reactive oligomers [J]. Macromol Mater Eng, 2000, 282:17-21.

TAN Guoxin, WANG Yingjun, LI Jun, et al. Synthesis and characterization of injectable photocrosslinking poly(ethylene glycol)diacrylate based hydrogels [J]. Polymer Bulletin, 2008, 61(1): 91-98.

郝星, 贺健康, 高琨,等. 蚕丝蛋白/明胶多孔肝组织支架的制备及性能研究[J]. 西安交通大学学报, 2011, 45(11): 121-126.

HAO Xing, HE Jiankang, GAO Kun,et al. Preparation and properties of silk fibroin/gelation porous scaffolds for liver tissue engineering [J]. Journal of Xi'an Jiaotong University, 2011, 45(11):121-126.

NAZAROV R, JIN H J, KAPLAN D L. Porous 3-D scaffolds from regenerated silk fibroin [J]. Biomacromolecules, 2004, 5(3): 718-726.

LEE Seung-Jae, KANG Taeyun, RHIE Jong-Won,et al. Development of three-dimensional hybrid scaffold using chondrocyte-encapsulated alginate hydrogel[J]. Sensors and Materials, 2007, 19(8): 445-451.

JACOBS P F. Fundamental processes: rapid prototyping and manufacturing: fundamentals of stereolithography[M]. Michigan,USA: Society of Manufacturing Engineers, 1992:79-110.

HOLLISTER S J, LIAO E E, MOFFITT E N, et al. Defining design targets for tissue engineering scaffolds; fundamentals of tissue engineering and regenerative medicine[M]. Berlin,Germany: Springer, 2009: 521-537.

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