马铃薯发酵产氢的菌株培养基改良研究

谢斌飞; 程军; 周俊虎; 宋文路; 刘建忠; 岑可法

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马铃薯发酵产氢的菌株培养基改良研究

氢能研究 | 更新时间：2025-12-08

- 马铃薯发酵产氢的菌株培养基改良研究
- Improvement of Bacteria Substrate for Hydrogen Production from Potatoes by Anaerobic Fermentation
- 西安交通大学学报 2008年42卷第6期页码：774-778
- 作者机构：
  
  浙江大学能源清洁利用国家重点实验室,杭州,310027
- 作者简介：
- 基金信息：
  
  : 2007-08-24. 作者简介: 谢斌飞(1981-),女,博士生;程军(联系人),男,教授,博士生导师. 基金项目: 国家自然科学基金资助项目(50406022,50525620);国家高技术研究发展计划资助项目(2006AA05Z122);教育部高等学校全国优秀博士学位论文专项基金资助项目(200437);霍英东高校青年教师基金资助项目(104021);浙江省科技攻关计划资助项目(2006C33049)
- DOI：
  中图分类号： X172
- 网络首发：2008-06-09，
  
  纸质出版：2008
- 稿件说明：
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谢斌飞, 程军, 周俊虎, 等. 马铃薯发酵产氢的菌株培养基改良研究[J]. 西安交通大学学报, 2008,42(6):774-778.

谢斌飞, 程军, 周俊虎, et al. Improvement of Bacteria Substrate for Hydrogen Production from Potatoes by Anaerobic Fermentation[J]. 2008, 42(6): 774-778.
谢斌飞, 程军, 周俊虎, 等. 马铃薯发酵产氢的菌株培养基改良研究[J]. 西安交通大学学报, 2008,42(6):774-778. DOI：

谢斌飞, 程军, 周俊虎, et al. Improvement of Bacteria Substrate for Hydrogen Production from Potatoes by Anaerobic Fermentation[J]. 2008, 42(6): 774-778. DOI：

摘要

为了促进马铃薯发酵产氢

研制了改良培养基驯化产氢菌株并使用淀粉生物酶降解马铃薯.将活性污泥煮沸后作为产氢菌株的接种体富集培养

使用Cl

代替SO

改良传统培养基消除了传统发酵产氢的启动滞留期(约12 h)

单位最大产氢速率从703.4 mL/(g·d)提高到800.5 mL/(g·d)

最大产氢潜力从205.2 mL/g增加到218.2 mL/g.通过α淀粉酶和糖化酶处理进一步提高了马铃薯的发酵产氢能力

单位最大产氢速率进一步提高到944.7 mL/(g·d)

最大产氢潜力进一步提高到267.2 mL/g.生物气中氢气的体积分数为43%～69%

无甲烷.

Abstract

In order to improve the hydrogen production from potatoes

the bacteria substrate was improved to domesticate H

-producing bacteria and potatoes are degraded by enzyme. The boiled activated sludge was used as H

-producing bacteria inoculum. While using the improved substrate

in which Cl

was used to substitute for SO

a lag-phase time(about 12 h)vanished

the maximum specific hydrogen production rate increased from 703.4 mL/(g·d)to 800.5 mL/(g·d)and the maximum hydrogen yield increased from 205.2 mL/g to 218.2 mL/g. When potatoes were pretreated by a amylase and glucoamylase

the hydrogen production was further enhanced. The maximum specific hydrogen production rate was then increased to 944.7 mL/(g·d

)and the maximum hydrogen yield was increased to 267.2 mL/g. It is found that the hydrogen composition of the biogas is about 43%～69% and no methane is detected.

关键词

Keywords

references

BOUALLAGUI H, TOUHAMI Y, BEN C, et al.Bioreactor performance in anaerobic digestion of fruit and vegetable wastes [J].Process Biochemistry,2005,40(3/4):989-995.

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KIM J K, OH B R, CHUN Y N, et al.Effects of temperature and hydraulic retention time on anaerobic digestion of food waste [J].Journal of Bioscience and Bioengineering,2006,102(4):328-332.

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KHANAL S K, CHEN W H, LI L, et al.Biological hydrogen production:effects of pH and intermediate products [J].International Journal of Hydrogen Energy, 2004, 29(11):1123-1131.

APHA.Standard methods for the examination of water and wastewater [M].19th ed. Washington DC,USA: American Public Health Association,1995.

林明,任南琪,马汐平,等.产氢发酵细菌培养基的选择和改进[J].哈尔滨工业大学学报,2003,35(4):398-402.

LIN Ming, REN Nanqi, MA Xiping,et al.Selection and improvement of culture for hydrogen producing bacteria [J].Journal of Harbin Institute of Technology, 2003, 35(4):398-402.

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