载细胞水凝胶体系中微通道结构对营养物输送能力的影响

黄国友 1

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载细胞水凝胶体系中微通道结构对营养物输送能力的影响

更新时间：2025-11-17

- 载细胞水凝胶体系中微通道结构对营养物输送能力的影响
- Influence of Microfluidic Channels on Delivery of Nutrient in Cell-Laden Hydrogels
- 西安交通大学学报 2011年45卷第4期页码：115-120
- 作者机构：
  
  西安交通大学生物医学工程与生物力学中心,西安,710049
- 作者简介：
- 基金信息：
  
  : 2010-10-13. 作者简介: 黄国友(1984-),男,博士生;卢天健(联系人),男,教授,博士生导师. 基金项目: 国家自然科学基金资助项目(10825210,10872157);国家“111引智计划”资助项目(B06024);陕西省自然科学基金资助项目(2010JQ1010);西安交通大学校基金资助项目(08143004)
- DOI：
  中图分类号： Q81
- 纸质出版：2011
- 稿件说明：
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黄国友 1. 载细胞水凝胶体系中微通道结构对营养物输送能力的影响[J]. 西安交通大学学报, 2011,45(4):115-120.

黄国友 1. Influence of Microfluidic Channels on Delivery of Nutrient in Cell-Laden Hydrogels[J]. 2011, 45(4): 115-120.
黄国友 1. 载细胞水凝胶体系中微通道结构对营养物输送能力的影响[J]. 西安交通大学学报, 2011,45(4):115-120. DOI：

黄国友 1. Influence of Microfluidic Channels on Delivery of Nutrient in Cell-Laden Hydrogels[J]. 2011, 45(4): 115-120. DOI：

摘要

为了优化载细胞水凝胶中微通道的结构设计

通过数值模拟

系统地研究了孔隙率、微通道数目及排布方式等参数对营养物输送能力的影响.研究结果表明:在给定微通道数目下

微通道尺寸和间距均存在最优值

与此相对应的营养物输送能力最佳; 当孔隙率或微通道数目较大时

微通道排布方式对营养物的输送能力无明显影响.通过参数优化

可以更好地提高微通道的营养物输送能力

尤其是保证远离微通道表面的细胞得到充足的营养物供应

为组织工程化组织中微通道结构的优化设计提供指导.

Abstract

To optimize the structural parameters of microfluidic cell-laden hydrogels embedded with microchannels

a mathematical model is developed to systematically investigate the influence of microchannel size

inter-channel separation

number of channels and their distribution on nutrient transport in cell-laden hydrogels. The simulation results show that there exist the optimal microchannel size and inter-channel separation for enhanced nutrient utilization. While the porosity is fixed

the capability of nutrient transport is improved with the increasing channel number. The distribution of channels exerts slight effect on the nutrient delivery as the number of channels gets beyond 6. In addition

the nutrient transport can be enhanced by adjusting the diffusion properties of hydrogels. The present results provide useful guidance for the experimental design of microfluidic tissue constructs.

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references

WOLFE R A, ROYS E C, MERION R M. Trends in organ donation and transplantation in the united states, 1999-2008 [J]. Am J Transplant, 2010, 10(4p2): 961-972.

LANGER R,VACANTI J P. Tissue engineering [J]. Science, 1993, 260(5110): 920-926.

KIRSNER R S, FALANGA V, EAGLSTEIN W H. The development of bioengineered skin [J]. Trends Biotechnol, 1998, 16(6): 246-249.

胡小红. 软骨修复用水凝胶的制备和性能研究 [D]. 杭州: 浙江大学高分子科学与工程学系, 2009.

SHIN'OKA T, IMAI Y, IKADA Y. Transplantation of a tissue-engineered pulmonary artery [J]. N Engl J Med, 2001, 344(7): 532-533.

PAPENBURG B J, LIU J, HIGUERA G A, et al. Development and analysis of multi-layer scaffolds for tissue engineering [J]. Biomaterials, 2009, 30(31): 6228-6239.

WU W, HANSEN C, ARAGON A, et al. Direct-write assembly of biomimetic microvascular networks for efficient fluid transport [J]. Soft Matter, 2010, 6(4): 739-742.

LING Y, RUBIN J, DENG Y, et al. A cell-laden microfluidic hydrogel [J]. Lab Chip, 2007, 7(6): 756-762.

LEE W, LEE V, Polio S, et al. On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels [J]. Biotechnol Bioeng, 2010, 105(6): 1178-1186.

SONG Y S, LIN R L, MONTESANO G, et al. Engineered 3D tissue models for cell-laden microfluidic channels [J]. Anal Bioanal Chem, 2009, 395(1): 185-193.

BIAN L, ANGIONE S L, NG K W, et al. Influence of decreasing nutrient path length on the development of engineered cartilage [J]. Osteoarthritis Cartilage, 2009, 17(5): 677-685.

CUCHIARA M P, ALLEN A C, CHEN T M, et al. Multilayer microfluidic PEGDA hydrogels [J]. Biomaterials, 2010, 31(21): 5491-5497.

RADISIC M, DEEN W, LANGER R, et al. Mathematical model of oxygen distribution in engineered cardiac tissue with parallel channel array perfused with culture medium containing oxygen carriers [J]. American Journal of Physiology-Heart and Circulatory Physiology, 2005, 288(3): H1278-H1289.

ZHOU S, CUI Z, URBAN J P G. Nutrient gradients in engineered cartilage: metabolic kinetics measurement and mass transfer modeling [J]. Biotechnol Bioeng, 2008, 101(2): 408-421.

NICHOLSON C. Diffusion and related transport mechanisms in brain tissue [J]. Rep Prog Phys, 2001, 64(7): 815-884.

GRAY W, LEE P. On the theorems for local volume averaging of multiphase systems [J]. Int J Multiphase Flow, 1977, 3: 333-340.

NICHOLSON C. Interaction between diffusion and Michaelis-Menten uptake of dopamine after iontophoresis in striatum [J]. Biophys J, 1995, 68(5): 1699-1715.

SENGERS B G, VAN DONKELAAR C C, OOMENS C W J, et al. Computational study of culture conditions and nutrient supply in cartilage tissue engineering [J]. Biotechnol Prog, 2005, 21(4): 1252-1261.

PATHI P, MA T, LOCKE B R. Role of nutrient supply on cell growth in bioreactor design for tissue engineering of hematopoietic cells [J]. Biotechnol Bioeng, 2005, 89(7): 743-758.

SENGERS B G, HEYWOOD H K, LEE D A, et al. Nutrient utilization by bovine articular chondrocytes: a combined experimental and theoretical approach [J]. J Biomech Eng-T Asme, 2005, 127(5): 758-766.

翁丽惠, 徐坚, 梁松苗, 等. 水凝胶中物质扩散过程研究进展 [J]. 高分子通报, 2005(5): 100-107.

WENG Lihui, XU Jian, LIANG Songmiao, et al. Progress in the research of the molecular diffusion in hydrogels[J]. Polymer Bulletin, 2005(5):100-107.

LI R H, ALTREUTER D H, GENTILE F T. Transport characterization of hydrogel matrices for cell encapsulation [J]. Biotechnol Bioeng, 1996, 50(4): 365-373.

GEANKOPLIS C.Transport processes: momentum, heat, and mass [M]. Newton, MA, USA: Allyn and Bacon, 1983:495-506.

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