Experimental Study on CO<sub>2</sub> Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of <italic style="font-style: italic">Chlorella vulgaris</italic>

WEI Xuan; YU Guiyuan; GUO Xiang; CAO Wen; GUO Liejin

doi:10.7652/xjtuxb202509012

您当前的位置：

首页 >

文章列表页 >

Experimental Study on CO₂ Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of Chlorella vulgaris

更新时间：2025-09-11

- Experimental Study on CO₂ Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of Chlorella vulgaris
- Journal of Xi’an Jiaotong University Vol. 59, Issue 9, Pages: 122-131(2025)
- 作者机构：
  
  西安交通大学动力工程多相流国家重点实验室， 710049，西安
- 作者简介：
- 基金信息：
- DOI：10.7652/xjtuxb202509012
  CLC： TK6
- Received：06 January 2025，
  
  Online First：15 April 2025，
  
  Published：10 September 2025
- 稿件说明：
移动端阅览
WEI Xuan, YU Guiyuan, GUO Xiang, et al. Experimental Study on CO₂ Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of Chlorella vulgaris[J]. Journal of Xi’an Jiaotong University, 2025, 59(9): 122-131. DOI: 10.7652/xjtuxb202509012.
DOI：

WEI Xuan, YU Guiyuan, GUO Xiang, et al. Experimental Study on CO₂ Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of Chlorella vulgaris[J]. Journal of Xi’an Jiaotong University, 2025, 59(9): 122-131. DOI: 10.7652/xjtuxb202509012. DOI：

摘要

针对微藻收获过程中化学絮凝剂成本高、环境风险大以及自絮凝效率较低的问题，系统研究了通过调控CO

体积分数诱导小球藻自絮凝的作用机制，重点探讨胞外有机物（EOM）在该过程中的关键调节作用。实验采用3种CO

体积分数（约0.04%、15%、25%）培养条件，通过分析EOM特性、pH和无机盐质量浓度等参数，综合评估了不同培养条件下小球藻的自絮凝行为及其影响因素。实验结果表明，CO

体积分数显著影响EOM的分泌量，进而改变小球藻的自絮凝效率。相较于CO

体积分数为15%和25%的培养组，CO

体积分数约为0.04%时培养的小球藻单位生物量分泌的蛋白质和多糖的质量浓度分别提高至其4~7倍和3~5倍，自絮凝效率最高达86%。去除EOM后，小球藻在12 h后的自絮凝效率下降了13%~24%，进一步验证了EOM在细胞自絮凝过程中的关键调控作用。相比之下，pH和无机盐质量浓度变化对自絮凝效率影响不显著。从CO

供给角度出发，系统揭示了CO

、EOM、自絮凝三者之间的关联机制，为低成本、高效率微藻的液固分离工艺的优化提供了实验参考。

Abstract

To address the challenges of high chemical flocculant costs

high environmental risks

and low self-flocculation efficiency in microalgae harvesting

this study systematically investigates the mechanism of inducing self-flocculation of

Chlorella vulgaris

by regulating the CO

concentration

with a focus on the key regulatory role of extracellular organic matter (EOM) in the process. Three CO

concentration cultivation conditions (about 0.04%

15%

and 25%) are employed

and parameters such as EOM characteristics

pH and concentrations of inorganic salts are analyzed to comprehensively evaluate the self-flocculation behavior of

Chlorella vulgaris

and its influencing factors under different cultivation conditions. The experimental results indicate that the CO

conce

ntration significantly affects the secretion of EOM

thus altering the self-flocculation efficiency of

Chlorella vulgaris

. Compared to the 15% and 25% CO

concentration cultivation groups

the protein and polysaccharide concentrations secreted per unit biomass of

Chlorella vulgaris

cultivated at approximately 0.04% CO

concentration increase by 4 to 7 times and 3 to 5 times

respectively

with the highest self-flocculation efficiency reaching 86%. After the removal of EOM

the self-flocculation efficiency of

Chlorella vulgaris

decreases by 13% to 24% after 12 hours

further validating the critical regulatory role of EOM in the self-flocculation process. In contrast

variations in pH and inorganic salt concentrations have a negligible effect on self-flocculation efficiency. From the perspective of CO

supply

this study reveals the interconnection mechanism among CO

EOM

and self-flocculation

providing experimental references for optimizing low-cost

high-efficiency liquid-solid separation processes for microalgae.

关键词

Keywords

references

HE Dahai , YIN Zhihong , QIN Lin , et al . The application of magical microalgae in carbon sequestration and emission reduction: removal mechanisms and potential analysis [J ] . Renewable and Sustainable Energy Reviews , 2024 , 197 : 114417 .

MENDONÇA I , FARIA M , RODRIGUES F , et al . Microalgal-based industry vs. microplastic pollution: current knowledge and future perspectives [J ] . Science of the Total Environment , 2024 , 909 : 168414 .

OUEDRHIRI W , BENNIS I , EL ARROUSSI H . Recent advances in microalgae-based vitamin D metabolome: biosynthesis, and production [J ] . Bioresource Technology , 2024 , 407 : 131078 .

王睿龙 , 李明佳 , KENTISH S . 碳酸酐酶附着膜在微藻直接空气碳捕集中的应用与固碳性能的强化 [J ] . 西安交通大学学报 , 2024 , 58 ( 9 ): 11 - 18 .

WANG Ruilong , LI Mingjia , KENTISH S . Application of carbonic anhydrase membrane in direct air carbon capture by microalgae and enhancement of carbon sequestration performance [J ] . Journal of Xi'an Jiaotong University , 2024 , 58 ( 9 ): 11 - 18 .

宋秉烨 , 李明佳 , 童自翔 , 等 . 碱性阴离子交换膜燃料电池用于消除微藻固碳过程中溶氧效应的实验研究 [J ] . 西安交通大学学报 , 2020 , 54 ( 8 ): 27 - 34 .

SONG Bingye , LI Mingjia , TONG Zixiang , et al . An experimental study on the dissolved oxygen consumption with alkaline anion-exchange membrane fuel cell [J ] . Journal of Xi'an Jiaotong University , 2020 , 54 ( 8 ): 27 - 34 .

SUMATHI Y , DONG Chengdi , SINGHANIA R R , et al . Advancements in nano-enhanced microalgae bioprocessing [J ] . Bioresource Technology , 2024 , 401 : 130749 .

MAGHZIAN A , ASLANI A , ZAHEDI R . A comprehensive review on effective parameters on microalgae productivity and carbon capture rate [J ] . Journal of Environmental Management , 2024 , 355 : 120539 .

RAO N R H , BEYER V P , HENDERSON R K , et al . Microalgae harvesting using flocculation and dissolved air flotation: selecting the right vessel for lab-scale experiments [J ] . Bioresource Technology , 2023 , 374 : 128786 .

MOLINA GRIMA E , BELARBI E H , ACIÉN FERNÁNDEZ F G , et al . Recovery of microalgal biomass and metabolites: process options and economics [J ] . Biotechnology Advances , 2003 , 20 ( 7/8 ): 491 - 515 .

赵雨枫 , 宋新山 , 曹新 , 等 . 不同壳聚糖改性黏土对小球藻的絮凝效应及絮凝条件优选 [J ] . 农业工程学报 , 2018 , 34 ( 16 ): 198 - 204 .

ZHAO Yufeng , SONG Xinshan , CAO Xin , et al . Flocculation effect and optimal flocculation conditions of Chlorella pyrenoidosa by different flocculants of modified chitosan and clay minerals [J ] . Transactions of the Chinese Society of Agricultural Engineering , 2018 , 34 ( 16 ): 198 - 204 .

SOUDAGAR M E M , KIONG T S , JATHAR L , et al . Perspectives on cultivation and harvesting technologies of microalgae, towards environmental sustainability and life cycle analysis [J ] . Chemosphere , 2024 , 353 : 141540 .

郑蕾 , 邢钰梓 , 宋建昕 , 等 . 磷源调控蛋白核小球藻胞外聚合物及生物絮凝性 [J ] . 中国环境科学 , 2023 , 43 ( 3 ): 1097 - 1106 .

ZHENG Lei , XING Yuzi , SONG Jianxin , et al . Phosphorus source regulates extracellular polymers and bioflocculation of Chlorella pyrenoidosa [J ] . China Environmental Science , 2023 , 43 ( 3 ): 1097 - 1106 .

CHEN Xin , LI Chengxuan , ZHANG Yiming , et al . Mechanism of rapid settling algal flocs formation in a novel fluidized bed flocculator [J ] . Algal Research , 2024 , 82 : 103605 .

FASAEI F , BITTER J H , SLEGERS P M , et al . Techno-economic evaluation of microalgae harvesting and dewatering systems [J ] . Algal Research , 2018 , 31 : 347 - 362 .

RAO N R H , BEYER V , THIELEMANS W , et al . Techno-economic comparison of flocculation combined with dissolved air flotation versus sedimentation for microalgae harvesting [J ] . Algal Research , 2024 , 81 : 103581 .

VAN DEN HENDE S , VERVAEREN H , BOON N . Flue gas compounds and microalgae: (bio-) chemical interactions leading to biotechnological opportunities [J ] . Biotechnology Advances , 2012 , 30 ( 6 ): 1405 - 1424 .

WEI Chaoyang , HUANG Yun , LIAO Qiang , et al . Analysis of the energy barrier between Chlorella vulgaris cells and their interfacial interactions with cationic starch under different pH and ionic strength [J ] . Bioresource Technology , 2020 , 304 : 123012 .

PENG Hongyan , HUANG Yun , XIA Ao , et al . Revealing mechanism and influence of microalgae cells' periodical auto-agglomeration induced by high concentration of carbon dioxide [J ] . Bioresource Technology , 2023 , 382 : 129120 .

CHEN Zhipeng , QIU Shuang , YU Ziwei , et al . Enhanced secretions of algal cell-adhesion molecules and metal ion-binding exoproteins promote self-flocculation of Chlorella sp. cultivated in municipal wastewater [J ] . Environmental Science & Technology , 2021 , 55 ( 17 ): 11916 - 11924 .

CHENG Shaozhe , ZHANG Haiyang , WANG Hailing , et al . Influence of algal organic matter in the in-situ flotation removal of Microcystis using positively charged bubbles [J ] . Bioresource Technology , 2024 , 397 : 130468 .

ZHOU Yun , CUI Xiaocai , WU Beibei , et al . Microalgal extracellular polymeric substances (EPS) and their roles in cultivation, biomass harvesting, and bioproducts extraction [J ] . Bioresource Technology , 2024 , 406 : 131054 .

NAVEED S , LI Chonghua , LU Xinda , et al . Microalgal extracellular polymeric substances and their interactions with metal(loid)s: a review [J ] . Critical Reviews in Environmental Science and Technology , 2019 , 49 ( 19 ): 1769 - 1802 .

FRØLUND B , GRIEBE T , NIELSEN P H . Enzymatic activity in the activated-sludge floc matrix [J ] . Applied Microbiology and Biotechnology , 1995 , 43 ( 4 ): 755 - 761 .

ZHANG Xiaoqi , BISHOP P L , KINKLE B K . Comparison of extraction methods for quantifying extracellular polymers in biofilms [J ] . Water Science and Technology , 1999 , 39 ( 7 ): 211 - 218 .

ŞIRIN S , SILLANPÄÄ M . Cultivating and harvesting of marine alga Nannochloropsis oculata in local municipal wastewater for biodiesel [J ] . Bioresource Technology , 2015 , 191 : 79 - 87 .

HANUMANTH RAO N R , YAP R , WHITTAKER M , et al . The role of algal organic matter in the separation of algae and cyanobacteria using the novel “Posi”-dissolved air flotation process [J ] . Water Research , 2018 , 130 : 20 - 30 .

TAKAARA T , SANO D , KONNO H , et al . Cellular proteins of Microcystis aeruginosa inhibiting coagulation with polyaluminum chloride [J ] . Water Research , 2007 , 41 ( 8 ): 1653 - 1658 .

WANG Qian , SHEN Qingyue , WANG Jixiang , et al . Insight into the rapid biogranulation for suspended single-cell microalgae harvesting in wastewater treatment systems: focus on the role of extracellular polymeric substances [J ] . Chemical Engineering Journal , 2022 , 430 ( Part 1 ): 132631 .

LIU Yanbo , ZHU Jing , LI Weirun , et al . Mechanism of membrane fouling mitigation by microalgae biofilm formation for low C/N mariculture wastewater treatment: EPS characteristics, composition and interfacial interaction energy [J ] . Journal of Environmental Chemical Engineering , 2024 , 12 ( 6 ): 114146 .

GE Shijian , CHAMPAGNE P . Nutrient removal, microalgal biomass growth, harvesting and lipid yield in response to centrate wastewater loadings [J ] . Water Research , 2016 , 88 : 604 - 612 .

LV Junping , GUO Baowen , FENG Jia , et al . Integration of wastewater treatment and flocculation for harvesting biomass for lipid production by a newly isolated self-flocculating microalga Scenedesmus rubescens SX [J ] . Journal of Cleaner Production , 2019 , 240 : 118211 .

BARBORÍKOVÁ J , ŠUTOVSKÁ M , KAZIMIEROVÁ I , et al . Extracellular polysaccharide produced by Chlorella vulgaris : chemical characterization and anti-asthmatic profile [J ] . International Journal of Biological Macromolecules , 2019 , 135 : 1 - 11 .

LAMA S , PAPPA M , BRANDÃO WATANABE N , et al . Interference of extracellular soluble algal organic matter on flocculation-sedimentation harvesting of Chlorella sp. [J ] . Bioresource Technology , 2024 , 411 : 131290 .

LIU Xuwei , RENARD C M G C , BUREAU S , et al . Revisiting the contribution of ATR-FTIR spectroscopy to characterize plant cell wall polysaccharides [J ] . Carbohydrate Polymers , 2021 , 262 : 117935 .

VANDAMME D , POHL P I , BEUCKELS A , et al . Alkaline flocculation of Phaeodactylum tricornutum induced by brucite and calcite [J ] . Bioresource Technology , 2015 , 196 : 656 - 661 .

黄敏 , 冯广鑫 , 朱素芹 , 等 . 碱法絮凝采收聚球藻7002的研究 [J ] . 安徽农业科学 , 2018 , 46 ( 18 ): 9 - 12 .

HUANG Min , FENG Guangxin , ZHU Suqin , et al . Alkaline flocculation for harvesting Synechococcus sp. PCC 7002 [J ] . Journal of Anhui Agricultural Sciences , 2018 , 46 ( 18 ): 9 - 12 .

RAO N R H , GONZALEZ-TORRES A , TAMBURIC B , et al . The influence of physical floc properties on the separation of marine microalgae via alkaline flocculation followed by dissolved air flotation [J ] . Algal Research , 2023 , 71 : 103024 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on the Thermodynamic Properties in the Anomalous Region of Supercritical Water

Integrated and Joint Design Optimization of Centrifugal Compressor Impeller and Casing Treatment at Multiple Working Points

Numerical Simulation of the Energy Conversion Process in Thermoacoustic Engines with Different Stack Materials

I/O Performance Modeling and Prediction Method for Large-Scale Parallel Applications Using Machine Learning

Diffusion Characteristics and Influencing Factors in Case of Hydrogen Leakage from Fuel Cell Vehicles Running in Tunnels

Related Author

No data

Related Institution

No data

⁰

Experimental Study on CO2 Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of Chlorella vulgaris

DOI：10.7652/xjtuxb202509012

摘要

Abstract

关键词

Keywords

references

Experimental Study on CO₂ Concentration-Regulated Extracellular Organic Matter Characteristics Inducing Self-Flocculation of Chlorella vulgaris