Fragmentation of Platymonas Subcordiformis in Anaerobic Environment and Effect on Hydrogenase Activity

彦飞 1; 2; 陈兆安 1; 陆洪斌 1; 虞星炬 1; 金美芳 1; 张卫 1

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Fragmentation of Platymonas Subcordiformis in Anaerobic Environment and Effect on Hydrogenase Activity

更新时间：2025-12-08

- Fragmentation of Platymonas Subcordiformis in Anaerobic Environment and Effect on Hydrogenase Activity
- Vol. 42, Issue 4, Pages: 497-500(2008)
- 作者机构：
  
  1. 中国科学院大连化学物理研究所,大连,116023
  2. 中国科学院研究生院,北京,100039
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q55
- Online First：10 April 2008，
  
  Published：2008
- 稿件说明：
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彦飞 1, 2, 陈兆安 1, et al. Fragmentation of Platymonas Subcordiformis in Anaerobic Environment and Effect on Hydrogenase Activity[J]. 2008, 42(4): 497-500.
DOI：

彦飞 1, 2, 陈兆安 1, et al. Fragmentation of Platymonas Subcordiformis in Anaerobic Environment and Effect on Hydrogenase Activity[J]. 2008, 42(4): 497-500. DOI：

摘要

为了分离纯化亚心型扁藻(Platymonas subcordiformis)体内的可溶性氢酶

研究了在厌氧环境下藻细胞的破碎方法

以及各种破碎条件对氢酶活性的影响.实验结果表明

使用超声法在输出功率为400 W、超声为5 s、间歇为10 s、工作20次的条件下

破碎细胞后溶液中总蛋白含量最高

可达2.73 g/L.珠磨法和体积分数为0.2%的Triton X-100法破碎的鲜细胞的氢酶提取效率较高

分别为1.44 U/g和1.27 U/g

其中使用Triton X-100法破碎后的粗提液所含其他蛋白的种类较少.实验的最终目的是获得足够量的氢酶进行下一步纯化

因此Triton X-100法是最合适的方法.

Abstract

To isolate and purify dissolvable hydrogenase in Platymonas subcordiformis cells

the scheme for alga cell fragmentation is designed in anaerobic environment

and the effects of experimental conditions on the hydrogenase activity are investigated. By means of ultrasonication

the concentration of total protein approaches to 2.73 g/L under the condition of 400 W output power

5 s crushing time

10 s interval for 20 times. The efficiency of hydrogenase isolation gets higher in case of glass beads or 0.2%(volume fraction)Triton X-100 to break up fresh cells to obtain 1.44 U/gand 1.27 U/g respectively

while the other proteins get less in crude extract by Triton X-100 method

which is suggested to gain enough purified hydrogenase.

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references

张卫,管英富,虞星炬,等.一种海洋绿藻两步法生物光解水制氢方法:中国,03110981.0 [P].2003-01-29.

BENEMANN J.Hydrogen biotechnology: progress and prospects[J]. Nature Biotechnol, 1996, 14:1101-1103.

de VALLADARES M R. IEA agreement on the production and utilization of hydrogen[R].Bethesda, USA: [s.n.],2003.

管英富,邓麦村,金美芳,等.微藻光生物水解制氢技术[J].中国生物工程杂志, 2003, 23(4):8-13.

GUAN Yingfu,DENG Maicun,JIN Meifang,et al. Advance in photo-biological hydrogen production by microalgae[J]. Progress in Biotechnology,2003,23(4):8-13.

CAROLYN C E. IEA agreement on the production and utilization of hydrogen[R]. Golden, USA: [s.n.], 1998.

GUAN Y F, ZHANG W, DENG M C, et al. Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis[J].Biotechnol Lett, 2004,26(13): 1031-1035.

RAN C Q, YU X J, MEI F J, et al. Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis[J]. Biotechnol Prog, 2006,22(2):1-6.

管英富. 绿藻间接光解水制氢过程的研究[D]. 大连: 中国科学院大连化学物理研究所, 2003.

ADAMS M W, HALL D O. Purification of the membrane-bound hydrogenase of Escherichia coli [J]. J Biochem, 1979, 183:11-22.

LOWRY O H, ROSEBROUGH N J, FARR A L, et al. Protein measurement with the Folin phenol reagent[J]. J Biol Chem, 1951, 194: 265-275.

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