Enhanced Hydrogen Photoproduction by Marine Green Microalga Platymonas subcordiformis Grown under CO<sub>2</sub>-Supplemented Air Bubble Bioreactor

郭祯 1; 2; 陈兆安 1; 陆洪斌 1; 虞星炬 1; 金美芳 1; 张卫 1

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Enhanced Hydrogen Photoproduction by Marine Green Microalga Platymonas subcordiformis Grown under CO₂-Supplemented Air Bubble Bioreactor

更新时间：2025-12-08

- Enhanced Hydrogen Photoproduction by Marine Green Microalga Platymonas subcordiformis Grown under CO₂-Supplemented Air Bubble Bioreactor
- Vol. 42, Issue 6, Pages: 779-783(2008)
- 作者机构：
  
  1. 中国科学院大连化学物理研究所,辽宁,大连,116023
  2. 中国科学院研究生院,北京,100039
- 作者简介：
- 基金信息：
- DOI：
  CLC： TQ116.2
- Online First：09 June 2008，
  
  Published：2008
- 稿件说明：
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郭祯 1, 2, 陈兆安 1, et al. Enhanced Hydrogen Photoproduction by Marine Green Microalga Platymonas subcordiformis Grown under CO₂-Supplemented Air Bubble Bioreactor[J]. 2008, 42(6): 779-783.
DOI：

郭祯 1, 2, 陈兆安 1, et al. Enhanced Hydrogen Photoproduction by Marine Green Microalga Platymonas subcordiformis Grown under CO₂-Supplemented Air Bubble Bioreactor[J]. 2008, 42(6): 779-783. DOI：

摘要

利用鼓泡式光生物反应器

比较通入空气及不同φ(CO

)(1%、3% 、5% 、10% 、15%)对亚心形扁藻生长及光合放氢的影响.实验结果表明:CO

含量对扁藻生长及光合放氢均有影响

其中φ(CO

)为3%时培养及光合放氢的效果最好

培养的藻细胞10 d细胞密度倍增3.8倍

比生长速率为0.134 d

-1

; 培养到第7 d

细胞密度调整为6×10

个/mL

暗诱导12 h后

在15 μmol/L解偶联剂羰基氰化物间氯苯腙(CCCP)作用下连续光照24 h

培养的藻细胞产氢量提高70%

最大比产氢速率为3.24 mmol/(g·h).代谢分析表明

φ(CO

)为3%时培养的藻细胞淀粉含量最高

是对照组的2.1倍

作为惟一碳源时淀粉含量与产氢量密切相关.利用CO

培养提高扁藻细胞产氢量的工艺有利于温室效应气体的减排.

Abstract

The kinetics of cell growth and photohydrogen evolution of P. subcordiformis grown under CO

-supplemented air bubble bioreactor is investigated. The highest cell growth was achieved as φ(CO

)got to 3%

and was supplemented to the air

with a specific growth rate of 0.134 d

-1

. The hydrogen photoproduction was enhanced by 70% for cells grown under 3% of φ(CO

)

with a specific hydrogen production rate of 3.24 mmol/(g·h)

-1

. The metabolic analysis of algal cells indicated a significant increase in starch accumu

lation with the increasing CO

concentrations. The highest starch content was achieved under 3% of φ(CO

)supplemented alga cells

2.1 times the control culture in air. The improved hydrogen production may correlate with the increased starch accumulation where CO

serves as the sole carbon source. These results suggest that hydrogen photoproduction by marine green alga can be beneficial from CO

-supplemented cultivation

which exerts a positive impact on the greenhouse gas mitigation.

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references

管英富,邓麦村,金美芳,等.微藻光生物水解制氢技术[J].中国生物工程杂志, 2003, 23(4):8-13.

GUAN Yingfu, DENG Maicun, JIN Meifang, et al. Advance in photo-biological hydrogen production by microalgae [J]. China Biotechnology, 2003,23(4):8-13.

GUAN Yingfu, DENG Maicun, YU Xingju, et al. Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis [J]. Biochem Eng J, 2004, 19(1): 69-73.

GUAN Yingfu, ZHANG Wei, DENG Maicun, et al. Significant enhancement of photobiological H2 evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis[J].Biotechnol Lett, 2004, 26(13):1031-1035.

RAN Chunqiu, YU Xingju, JIN Meifang, et al. Role of carbonyl cyanide m-chlorophenylhydrazone in enhancing photobiological hydrogen production by marine green alga Platymonas subcordiformis[J]. Biotechnol Prog, 2006, 22(2): 1-6.

张卫,郭祯,陈兆安,等.一种利用气体中二氧化碳使藻类快速增殖直接用于生物制氢的方法:中国,200710010804.X. [P].2001-03-30.

SATON A, KURANO N, MIYACHI S. Inhibition of photosynthesis by intracellular carbonic anhydrase in microalgae under excess concentrations of CO2[J]. Photosynth Res, 2001, 68(3): 215-224.

YOON J H, SIM S J, KIM M S, et al. High cell density culture of Anabaena variabilis using repeated injections of carbon dioxide for the production of hydrogen[J]. Int J Hydrogen Energy, 2002, 27(11): 1265-1270.

KOSOUROV S, PATRUSHEVA E, GHIRARDI M L, et al. A comparison of hydrogen photoproduction by sulfur-deprived Chlamydomonas reinhardtii under different growth condition [J]. J Biotech, 2007, 128(4): 776-787.

KRUSE O, RUPPRECHT J, BADER K P, et al. Improved photobiological H2 production in engineered green algal cells[J]. J Biol Chem, 2005, 280(40):34170-34177.

ANTAL T K, KRENDELEVA T E, LAURINAVICHENE T V, et al. The relationship between the photosystem 2 activity and hydrogen production in sulfur deprived Chlamydomonas reinhardtii cells[J]. Dokl Biochem Biophys, 2001, 381: 371-374.

KOSOUROV S, SEIBERT M, GHIRARDI M L. Effects of extracellular pH on the metabolic pathways in sulfur-deprived, H2-producing Chlamydomonas reinhardtii cultures[J].Plant Cell Physiol, 2003, 44(2):146-155.

ZHANG L, HAPPE T, MELIS A. Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii(green alga)[J]. Planta, 2002, 214(4): 552-561.

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⁰

Enhanced Hydrogen Photoproduction by Marine Green Microalga Platymonas subcordiformis Grown under CO2-Supplemented Air Bubble Bioreactor

DOI：

摘要

Abstract

关键词

Keywords

references

Enhanced Hydrogen Photoproduction by Marine Green Microalga Platymonas subcordiformis Grown under CO₂-Supplemented Air Bubble Bioreactor