Micro Fluidic Amperometric Immunosensor Chip for HIV p24 and gp36 Antigens Based on Gold Magnetic Nanoensemble Electrode

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Micro Fluidic Amperometric Immunosensor Chip for HIV p24 and gp36 Antigens Based on Gold Magnetic Nanoensemble Electrode

更新时间：2025-07-09

- Micro Fluidic Amperometric Immunosensor Chip for HIV p24 and gp36 Antigens Based on Gold Magnetic Nanoensemble Electrode
- Vol. 43, Issue 7, Pages: 115-119(2009)
- 作者机构：
  
  宁波大学宁波市新型功能材料及其制备科学国家重点实验室培育基地,浙江,宁波,315211
- 作者简介：
- 基金信息：
- DOI：
  CLC： O657.1
- Online First：10 July 2009，
  
  Published：2009
- 稿件说明：
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Micro Fluidic Amperometric Immunosensor Chip for HIV p24 and gp36 Antigens Based on Gold Magnetic Nanoensemble Electrode[J]. 2009, 43(7): 115-119.
DOI：

Micro Fluidic Amperometric Immunosensor Chip for HIV p24 and gp36 Antigens Based on Gold Magnetic Nanoensemble Electrode[J]. 2009, 43(7): 115-119. DOI：

摘要

研制了以聚碳酸酯为基底的双通道安培检测微流控免疫芯片

在其中分别集成了修饰有HIV核心抗原p24/gp36的单克隆一级抗体的纳米金修饰电极

并应用于人血清中p24和gp36抗原的同时分析.检测原理是:基于夹心免疫分析法

在电压驱动下

一次性加入含有p24和gp36样品及HRP酶标记的p24和gp36二级抗体溶液

与电极表面的一级抗体生成夹心免疫复合物; 接着在体系中加入H

以方波溶出伏安法检测免疫复合物上HRP催化H

的还原电流.在pH 为6.2的磷酸缓冲液中

对HIV p24和gp36的检测时间均少于2 min

线性范围为0.5～400 μg/L

检测限为0.25 μg/L.该芯片集成了加样、分离和检测系统

检测时间短

灵敏度明显高于传统的酶联免疫吸附分析法

可应用于对艾滋病的早期诊断和大范围筛查.

Abstract

An electrochemical detection biosensor chip is developed for simulately analyzing HIV p24 and gp36 antigens with gold nanoensemble electrode modified by the first monoclonal antibodies of p24 and gp36 antigens and microfluidic control system. The detection principle is based on double-sandwich immunoassay method. The first monoclonal antibodies of p24 and gp36 antigens are fixed on electrode surface

then when the serum containing p24 and gp36 antigens passes through the modified electrode

p24 and gp36 combine with the corresponding antibodies on its surface. Furthermore

the secondary p24 and gp36 antibodies marked by HRP are added to form a sandwich compound. Square wave stripping voltammetry is employed to determine the red

uction current by catalyzing H

. In pH 6.2 PBS

HIV p24 and gp36 can be detected within 2 minutes with a linear range on calibration curve from 0.5 to 400 μg/L and a detection limit of 0.25 μg/L. This biosensor improves the detective sensitivity of p24 and gp36 to 1 A·L·g

-1

and is expected to be chosen for early diagnosis of AIDS and large-scale screening test.

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references

LUKACS Z, DIETRICH A, GANSCHOW R, et al. Simultaneous determination of HIV antibodies, hepatitis C antibodies, and hepatitis B antigens in dried blood spots: a feasibility study using a multi-analyte immunoassay [J]. Clin Chem Lab Med, 2005, 43(2): 141-145.

SUSANNE P, HEDWIG D, FRANK L. A new combined HIV p24 antigen and anti-HIV-1/2/O screening assay [J]. Journal of Molecular Biology, 2005, 304: 229-243.

RAVANSHAD M, SABAHI F, MAHBOUDI F, et al. Evaluation of a new dot blot assay for confirmation of human immunodeficiency virus type 1 and 2 infections using recombinant p24, gp41, gp120 and gp36 antigens [J]. Journal of Saudi Medicine, 2006, 27(1): 31-36.

TONISCH K, CIMALLA V, WILL F, et al. Nanowire-based electromechanical biomimetic sensor [J]. Physica: E, 2007, 37(13): 208-211.

LIU Guodong, WANG Joseph, KIM J, et al. Electrochemical coding for multiplexed immunoassays of proteins [J]. Analytical Chemistry, 2004, 76(23): 7126-7130.

QIN Jianhua, AARON R W. Maze exploration and learning in C elegans [J]. Lab on a Chip, 2007, 7(12): 186-192.

YU Heng, CHEN Min, RICE P M. Dumbbell-like bifunctional Au-Fe3O4 nanoparticles [J]. Nano Letter, 2005, 5(2): 379-382.

WILSON M S. Electrochemical immunosensors for the simultaneous detection of two tumor markers [J]. Analytical Chemistry, 2005, 77(22): 1496-1502.

激光照射金纳米微粒靶向细胞对细胞膜通透性的影响. 2008, 42(12): 1560-1564.

铁掺杂纳米二氧化钛介孔材料的合成、结构与性能. 2008, 42(9): 1184-1188.

磁性交联核酸酶P1聚集体的制备及性质研究. 2008, 42(8): 1035-1039.

氧化锌/银双层膜负反馈阴极电极的制备及性能研究. 2007, 41(12): 1470-1473.

蒽甲醇修饰单壁碳纳米管的研究. 2007, 41(6): 746-749.

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