Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode

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Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode

更新时间：2025-07-09

- Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode
- Vol. 44, Issue 7, Pages: 114-119(2010)
- 作者机构：
  
  新型功能材料及其制备科学国家重点实验室, 宁波大学材料科学与化学工程学院,浙江,宁波,315211
- 作者简介：
- 基金信息：
- DOI：
  CLC： O657.1
- Online First：10 July 2010，
  
  Published：2010
- 稿件说明：
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Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode[J]. 2010, 44(7): 114-119.
DOI：

Amperometric Immunosensor for Human Chorionic Gonadotrophin Based on Antibody Coated Nano Gold Multilayer Modified Electrode[J]. 2010, 44(7): 114-119. DOI：

摘要

研制了一种采用人绒毛膜促性腺激素抗体(anti-HCG)包被巯基丁二酰胺(HL)/纳米金(AuNPs)多层膜修饰金电极的免疫传感器(Au|{HL/AuNPs}

-anti-HCG)

用于检测人唾液中的HCG.通过层层自组装技术在Au电极表面交替修饰多层{HL/AuNPs}

膜

再通过膜内的AuNPs吸附anti-HCG

构筑了HCG安培免疫传感器.以K

Fe(CN)

为探针

在pH 7.0的PBS缓冲液中加入HCG

随着HCG与电极表面anti-HCG发生特异性免疫反应

形成的抗原/抗体免疫复合物使得K

Fe(CN)

在电极表面的响应电流减小

减小量(I

)与膜表面的免疫反应程度相关

以此作为HCG定量的依据.对各修饰电极表面进行了表征和定量分析.实验表明:该免疫传感电极中{HL/AuNPs}

膜内存在的大量AuNPs显著增加了anti-HCG吸附量

从而使得其HCG检测范围变宽(0.1～100 mg/L)

检出下限为0.056 mg/L(3σ).

Abstract

An amperometric immunosensor for human chorionic gonadotrophin(HCG)in the saliva is developed based on HCG antibody(anti-HCG)coated mercapto succinic amide(HL)/gold nanoparticles(AuNPs)multilayer membrane modified Au electrode. HL/AuNPs monolayers are modified alternatively and form multiple membranes through Au-NH

and Au-S bonds by layer-by-layer self-assembly technology. K

Fe(CN)

is employed as electron probe for detection. The antigen-antibody immune complex formed on the electrode surface is able to hinder electron transport

so the redox current of K

Fe(CN)

is decreased. The decreased value of ΔI

is related to the extent of immune-reaction on membrane surface

which serves as the quantitative basis of HCG content. The experimental results show that multilayer AuNPs membrane can adsorb more anti-HCG

and obviously broaden the linear detection range of HCG to 0.1-100 mg/L with detection limit of 0.056 mg/L(3σ).

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references

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