Nano-Scale Photoporation by Tightly Focused Lasers: A Survey

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Nano-Scale Photoporation by Tightly Focused Lasers: A Survey

更新时间：2025-07-09

- Nano-Scale Photoporation by Tightly Focused Lasers: A Survey
- Vol. 46, Issue 10, Pages: 107-115(2012)
- 作者机构：
  
  1. 西安交通大学生命科学与技术学院,西安,710049
  2. 西安交通大学生物医学信息工程教育部重点实验室,西安,710049
- 作者简介：
- 基金信息：
- DOI：
  CLC： O427;O437;Q631
- Online First：10 October 2012，
  
  Published：2012
- 稿件说明：
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Nano-Scale Photoporation by Tightly Focused Lasers: A Survey[J]. 2012, 46(10): 107-115.
DOI：

Nano-Scale Photoporation by Tightly Focused Lasers: A Survey[J]. 2012, 46(10): 107-115. DOI：

摘要

论述了一种新型的细胞转导方法——纳米尺度激光紧聚焦光穿孔技术.按照光穿孔可能的物理机制

分4类讨论了现在主流的光穿孔技术:连续激光光穿孔

其中最前沿的技术是紫外二极管激光光穿孔; 纳秒、皮秒激光脉冲和经放大的千赫兹飞秒激光脉冲光穿孔

其中应用范围最广的是Nd:YAG激光器; 飞秒激光振荡器发出的兆赫兹脉冲序列光穿孔

该技术在多尺度、跨学科及在体研究中扮演重要角色; 最新出现的利用双脉冲微射流进行光穿孔

这是光穿孔技术中仅有的一种将外源物主动引入细胞内的方法.可以预见:当激光紧聚焦技术结合了双光子荧光显微镜、微芯片实验室、膜片钳技术等各种单细胞分析技术后

必将在细胞间通信、干细胞谱系提交、癌症转移、激光基因疗法、胚胎发育学以及神经功能再生等学科领域中产生重大影响.

Abstract

A novel cellular transfer method

nano-scale photoporation by tightly focused laser

is introduced. According to the underlying physical mechanisms

four main-stream photoporation methods are discussed-Photoporation by CW lasers

whose cutting edge technology is photoporation by violet diode laser; Photoporation with nanosecond

picosecond or amplified kHz femtosecond laser pulses

whose most commonly used laser is Nd:YAG laser; Photoporation based on MHz femtosecond pulse series from femtosecond oscillator

which plays a big role in multi-scale

interdisciplinary and in vivo research; The newly developed photoporation based on micro-jet induced by two pulses

which is regarded as the unique active photoporation method. It is predicted that photoporation

combined with the other techniques

such as two-photon microscope

lab-on-a-chip

catch-clamp

can strongly affect cell-cell communication

stem cell lineage commitment

cancer metastasis

laser gene therapy

embryology and nerve regeneration.

关键词

Keywords

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