Germicidal Efficiency and Mechanisms of Escherichia Coli by Remote Oxygen Plasma

刘红霞; 陈杰瑢; 周媛

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Germicidal Efficiency and Mechanisms of Escherichia Coli by Remote Oxygen Plasma

更新时间：2025-12-08

- Germicidal Efficiency and Mechanisms of Escherichia Coli by Remote Oxygen Plasma
- Vol. 42, Issue 1, Pages: 96-100(2008)
- 作者机构：
  
  西安交通大学环境科学与工程系,西安,710049
- 作者简介：
- 基金信息：
- DOI：
  CLC： R18
- Online First：10 January 2008，
  
  Published：2008
- 稿件说明：
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刘红霞, 陈杰瑢, 周媛. Germicidal Efficiency and Mechanisms of Escherichia Coli by Remote Oxygen Plasma[J]. 2008, 42(1): 96-100.
DOI：

刘红霞, 陈杰瑢, 周媛. Germicidal Efficiency and Mechanisms of Escherichia Coli by Remote Oxygen Plasma[J]. 2008, 42(1): 96-100. DOI：

摘要

采用Langmuir双电子探针和电子自旋共振诊断技术分别定量测定了远程氧等离子体场中各活性物种的分布.考察了远程氧等离子体对医用聚四氟乙烯表面大肠杆菌的灭活作用

并通过对灭菌后菌悬液蛋白质泄漏量、脂质过氧化物生成量及细菌DNA电泳图的测定分析了其灭菌机理.结果表明:远程氧等离子体中的电子、离子浓度随远程距离的增大迅速降低

30 cm后接近于0

而自由基浓度则相对降低缓慢

40 cm处仍为初始浓度的70%; 远程氧等离子体60 s内的灭菌效果值在放电区可高达3.42

在远程距离40 cm以内也均在3.32以上

符合消毒灭菌的要求; 电子、离子对细菌细胞膜的快速刻蚀及自由基对胞膜中多价不饱和脂肪酸的攻击分别是放电区及远程30 cm后灭菌的主要原因.氧等离子体中紫外光的灭菌效果微弱.

Abstract

Double Langmuir electron probes and electron spin resonance(ESR)were used to measure the distribution of electrons

ions and free radicals in remote oxygen plasma. The germicidal effect(GE)of Escherichia coli on the surface of medical polytetrafluoroethylene(PTFE)film by remote oxygen plasma was investigated

and then

the quantity of protein leakage and the production of lipid peroxide in bacterial suspension as well as DNA agarose electrophoresis were measured after sterilization to analyse the inactivation mechanisms. The results show that in remote oxygen plasma

the concentrations of electrons and ions decrease rapidly with the increasing distance from the center of induction coil

which approach to zero at 30 cm

whereas the concentration

of free radicals reduces slowly

namely

decrease 30% within 40 cm. GE reaches 3.42 in discharge zone(0 cm)and exceeds 3.32 within 40 cm when plasma treatment parameters are set as plasma RF power at 100 W

treatment time at 60 s and oxygen flux at 40 cm

/min. Fast etching action on cell membrane by electrons

ions and attacking polyunsaturation fatty acid(PUFA)in cell membrane by free radicals are the primary reasons of oxygen plasma sterilization in discharge and remote zone

respectively. The GE of UV radiation in remote oxygen plasma gets feeble.

关键词

Keywords

references

LAROUSSI M, LEIPOLD F. Evaluation of the roles of reactive species, heat, and UV radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure [J]. International Journal of Mass Spectrometry, 2004, 233: 81-86.

顾春英, 薛广波. 等离子体消毒和灭菌 [J]. 上海预防医学杂志, 1999, 11(11): 486-491.

GU Chunying, XUE Guangbo. Plasma disinfection and sterilization [J]. Shanghai Journal of Prevention Medicine, 1999, 11(11): 486-491.

LEROUGE S, WERTHEIMER M R, YAHIA L H. Plasma sterilization: a review of parameters, mechanisms, and limitations [J]. Plasma and Polymers, 2001, 6(3): 175-188.

OSTRIKOV K N, DENYSENKO I B, TSAKADZE E L. Diagnostics and two-dimensional simulation of low-frequency inductively coupled plasmas with neutral gas heating and electron heat fluxes [J]. Journal of Applied Physics, 2002, 92(9): 4935-4946.

LI Yong, WANG Qi, GUO Jinliang. ESR studies on the interaction of calixarenes and free radicals [J]. Materials Science and Engineering, 1999, C10: 25-28.

张文福. 医学消毒学 [M]. 北京:军事医学科学出版社,2002: 287-295.

CHANTAL E H, CHENG C, JOHN E D, et al. Optimizing the sterilization of PLGA Scaffolds for use in tissue engineering [J]. Biomaterials, 2001, 22: 25-31.

PUREVDORJ D, IGURA N, HAYAKAWA I, et al. Inactivation of escherichia coli by microwave induced low temperature argon plasma treatments [J]. Journal of Food Engineering, 2002, 53: 341-346.

SAMBROOK J, LASSELL D W. 分子克隆实验指南 [M]. 黄培堂,译. 北京:科学出版社,2002: 389-395.

CHEN Jierong, YAN Jinglian, ZHANG Yunze. Surface modification of medical PVC by remote oxygen plasma [J]. Composite Interfaces, 2004, 11(2): 123-130.

GOLDMAN A, AMOUROUX J. Plasma chemistry [C]∥KUNHARDT E E, LUSSENL H. Electrical Breakdown and Discharge in Gases, Macroscopic Processes and Discharge. New York: Plenum, 1983: 293-346.

KO T M, COOPER S L. Surface properties and platelet adhesion characteristics of acrylic acid and allylamine plasma treated polyethylene [J]. Journal of Applied Polymer Science, 1993, 47(1): 1601-1619.

BOL'SHAKOV A A, CRUDEN B A, MOGUL R, et al. Radio-frequency oxygen plasma as a sterilization source [J]. AIAA Journal, 2004, 42(4): 823-832.

SOLOSHENKO I O, KHOMICH V A, TSIOLKO V V, et al. Experimental and theoretical investigation of cold sterilization of medical instruments by plasma DC glow discharge [C]∥International Conference on Spectroscopy of Molecules and Crystals. Bellingham,Washington: SPIE, 2002: 308-314.

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刘红霞 1

张慧君 1

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Related Institution

School of Energy and Power Engineering, Xi'an Jiaotong University

State Key Laboratory of Multiphase Flow in Power Engineering, Xi'an Jiaotong University

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