Preparation and Property of Magnetic Cross-Linked Nuclease P1 Aggregations

邵卫祥; 莫晓燕; 李黎

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Preparation and Property of Magnetic Cross-Linked Nuclease P1 Aggregations

更新时间：2025-12-08

- Preparation and Property of Magnetic Cross-Linked Nuclease P1 Aggregations
- Vol. 42, Issue 8, Pages: 1035-1039(2008)
- 作者机构：
  
  西安交通大学生命科学与技术学院,西安,710049
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q814.2
- Online First：10 August 2008，
  
  Published：2008
- 稿件说明：
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邵卫祥, 莫晓燕, 李黎. Preparation and Property of Magnetic Cross-Linked Nuclease P1 Aggregations[J]. 2008, 42(8): 1035-1039.
DOI：

邵卫祥, 莫晓燕, 李黎. Preparation and Property of Magnetic Cross-Linked Nuclease P1 Aggregations[J]. 2008, 42(8): 1035-1039. DOI：

摘要

采用磁性纳米颗粒与酶蛋白共沉淀后经戊二醛交联的方法

制备了磁性交联核酸酶P1聚集体

并且对比分析了游离酶和固定化酶的部分酶学性质.优化的最佳制备条件为:硫酸铵质量浓度为0.8 g/mL

沉淀时间为0.5 h

戊二醛体积分数为0.6%

交联时间为2 h

所制得的固定化酶活性回收率为32.4%.酶学性质研究表明

固定化酶的K

值(30.7 mmol/L)明显高于游离酶的(7.27 mmol/L)

二者最适反应温度分别为90 ℃和75 ℃

最适pH值均为5.2

固定化核酸酶P1对热和酸碱的耐受性明显增强

连续反应6次后酶活力仍保留70%

良好的操作稳定性和磁响应性有利于核酸酶P1的工业化应用.

Abstract

The magnetic cross-linked enzyme aggregations(CLEAs)were prepared by depositing of nuclease P1 with magnetic nanoparticles and cross-linked with glutaraldehyde

and some enzymetic properties were compared between nuclease P1 and CLEAs. The optimal conditions of preparing magnetic CLEAs are sought out:(NH

)

80% saturation

depositing time 0.5 h

glutaraldehyde 0.6%

and cross-linking time 2 h. The activity recovery reaches to 32.4% under the above conditions. Investigation of enzymetic properties suggests that K

of magnetic CLEAs(30.7 mmol/L)gets higher than soluble enzyme(7.27 mmol/L)

and the optional reacting temperature of immobilized nuclease P1 is appropriate at 90 ℃

while optional reacting pH approaches to 5.2. Once immobilized

the resistance to heat

acid and alkali of nuclease P1 is remarkably improved

and the magnetic CLEAs maintained 70% activity after six consecutive reactions. Good operational stability and magnetic response are beneficial for industrial utilization of nuclease P1.

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references

武仙山,何立千,叶磊.交联酶聚集体:一种无载体酶固定化方法[J].生物技术,2005, 15(2):90-92.

WU Xianshan HE Liqian YE Lei. Cross-linked enzyme aggregations: an immobilizing method without carrier [J]. Biotechnology, 2005, 15(2):90-92.

任广智,李振华,何炳林.磁性高分子微球用于固定化酶的研究进展[J].离子交换与吸附,2000,16(1):83-87.

REN Guangzhi,LI Zhenhua,HE Binglin.Advances in enzyme immobilization using magnetic polymermicrospheres[J].Ion Exchange and Adsorption,2000,16(1):83-87.

廖红东,莫晓燕.核酸酶P1研究进展[J].药物生物技术,2004,11(3):190-193.

LIAO Hongdong,MO Xiaoyan.Research progress on nuclease P1[J]. Pharmaceutical Biotechnology,2004,11(3):190-193.

任欢鱼,刘蕾,刘勇键.磁流体的制备与性质研究[J].中国粉体技术,2003, 9(1):21-23.

REN Huanyu,LIU Lei,LIU Yongjian. Preparation and properties of magnetic fluid[J].China Powder Science and Technology,2003,9(1):21-23.

莫晓燕,宋威,张芹,等.桔青霉生产核酸酶P1发酵条件优化研究[J].西安交通大学学报,2003, 37(10):1083-1086.

MO Xiaoyan,SONG Wei,ZHANG Qin.Study on the optimization of fermentation conditions for penicillium citrinum producing nuclease P1[J]. Journal of Xi'an Jiaotong University,2003,37(10):1083-1086.

LORENA W, LORENA B, GLORIA F, et al. Cross-linked aggregates of multimeric enzymes:a simple and efficient methodology to stabilize their quaternary structure[J]. Biomacromolecules, 2004(5): 814-817.

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