Dielectric Property Investigation for Biological Cells with Electrokinetic Method

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Dielectric Property Investigation for Biological Cells with Electrokinetic Method

更新时间：2025-07-09

- Dielectric Property Investigation for Biological Cells with Electrokinetic Method
- Vol. 45, Issue 6, Pages: 85-90(2011)
- 作者机构：
  
  西安交通大学电力设备电气绝缘国家重点实验室,西安,710049
- 作者简介：
- 基金信息：
- DOI：
  CLC： TM930.12
- Online First：10 June 2011，
  
  Published：2011
- 稿件说明：
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Dielectric Property Investigation for Biological Cells with Electrokinetic Method[J]. 2011, 45(6): 85-90.
DOI：

Dielectric Property Investigation for Biological Cells with Electrokinetic Method[J]. 2011, 45(6): 85-90. DOI：

摘要

采用软胶粒动电理论并结合悬浮细胞单元的双电层模型

研究了在超声作用下细胞悬液的振动电位模型

并分别给出了离子和细胞动电极化电位的数学表达式及其之间的关系.根据模型建立了基于阶跃超声信号的细胞悬液动电测试系统.通过对NaCl溶液的振动电位测量表明

在低浓度离子溶液中

离子振动电位与离子浓度成正比

当离子浓度升高到一定值后

离子振动电位随离子浓度的升高呈波浪形下降

并由于超声凝聚颗粒的动电极化作用

会反相增加.通过对鸡血细胞悬液的振动电位测量表明

在低浓度细胞悬液中

由于离子与细胞动电极化的相互影响

悬液的振动电位随细胞浓度的增加而减小

当细胞浓度升高到6×10

-1

时

随着细胞浓度的升高

悬液的振动电位开始升高

此后鸡血细胞的动电极化对悬液振动电位的升高起到主要作用

从转折后的动电曲线的斜率可以实现对细胞介电特性的研究.

Abstract

Following the electrokinetic theory of soft particle and the double-layer model of suspended cell unit

the colloid vibration potential(CVP)model of biological cell under the action of ultrasound is investigated

and the mathematical expression and the interaction of the electrokinetic polarization potential of ion and cell are constructed. Based on the electrokinetic model of the cell suspension

an electrokinetic measurement system using the step ultrasonic signal is set up. Via the vibration potential measurement of the solution of NaCl

it shows that the ion vibration potential(IVP)gets direct proportional to the ion concentration. But the IVP wavily drops with the increasing ion concentration once the ion concentration increases to a certain level

or even increases with reversed-phase due

to the action of the electrokinetic polarization of particle of ultrasonic condensation. The vibration potential measurement of the chicken blood cell suspension shows that the total vibration potential(TVI)of the cell suspension decreases with the increasing cell concentration in the cell suspension in low concentration. When the cell concentration gets up to 6×10

-1

the TVI begins to increase with the increasing cell concentration

henceforth

the electrokinetic polarization of cell primarily facilitates TVI increase.

关键词

Keywords

references

刘亚利, 黄维, 高恩泉, 等. 小鼠肾近曲小管上皮细胞顶侧膜钾通道的膜片钳研究[J]. 第三军医大学学报, 2006, 28(1): 44-46.

LIU Yali, HUANG Wei, GAO Enquan, et al. Study of K～(+)channels at the apical membrane of mouse renal proximal tubule cell with patch-clamp [J]. Acta Academiae Medicinae Militaris Tertiae, 2006, 28(1): 44-46.

董玥, 朱永春, 高鸿雁, 等. 酵母菌单细胞电化学行为的循环伏安法及电化学交流阻抗研究[J]. 沈阳师范大学学报:自然科学版, 2010, 28(2): 251-254.

DONG Yue, ZHU Yongchun, GAO Hongyan,et al. Electrochemical behavior of single yeast cell by cyclic voltammetry and electrochemical impedance spectroscopy[J]. Journal of Shenyang Normal University:Natural Science Edition,2010, 28(2): 251-254.

SLUSARCZYK K, DANIEI M, SZERMER M, et al. Simulating and modeling electrophoresis phenomenon in capillaries [C]∥Proceedings of the International Conference on Mondern Problems Science. Lviv, Ukraine: National University Lviv Polytechnic, 2002:363-364.

周永昌,郭万学.超声医学[M]. 北京:科学文献出版社, 2006:65-67.

HILL R J, SAVILLE D A. ‘Exact' solutions of the full electrokinetic model for soft spherical colloids: electrophoretic mobility [J]. Colloids and Surfaces: A Physicochem Eng Aspects, 2005, 267(2): 31-49.

DUKHIN A S, GOETZ P J, VAN DE VEN T G. Ultrasonic characterization of proteins and blood cells [J]. Colloids and Surfaces: B Biointerfaces, 2006, 53(2): 121-126.

OHSHIMA H. Colloid vibration potential in a suspension of soft particles [J]. Colloids and Surfaces: A Physicochem Eng Aspects, 2007, 306(2): 83-87.

OHSHIMA H. Colloid vibration potential in a suspension of spherical colloidal particles [J]. Colloids and Surfaces: B Biointerfaces, 2007, 56(1): 16-18.

OHSHIMA H. Electrokinetic phenomena in a concentrated suspension of soft particles [J]. Colloids and Surfaces: A Physicochemical and Engineering Aspects, 2001, 195(1): 129-134.

DELGADO A V, GONZÁLEZ-CABALLERO F, HUNTER R J,et al. Measurement and interpretation of electrokinetic phenomena [J]. Journal of Colloid and Interface Science, 2007, 309(2): 194-224.

GREENWOOD R. Review of the measurement of zeta potentials in concentrated aqueous suspensions using electroacoustics [J]. Advances in Colloid and Interface Science, 2003, 106(2): 55-81.

YAN Yinghe, LEE Eric. Electrophoresis in concentrated dispersions of charged porous spheres [J]. Chemical Engineering Science, 2008, 63(23): 5719-5727.

O'BRIEN R W. The electroacoustic equations for a colloidal suspension [J]. J Fluid Mech, 1990, 212(1): 81-93.

O'BRIEN R W, MIDMORE B R, LAMB A,et al. Electroacoustic studies of moderately concentrated colloidal suspensions [J]. Faraday Disc, 1990, 90(1): 301-312.

DUKHIN A S, GOETZ P J. New developments in acoustic and electroacoustic spectroscopy for characterizing concentrated dispersions [J]. Colloids and Surfaces: A Physicochemical and Engineering Aspects, 2001,192(3): 267-306.

CARSTENSEN E L, LI Kam, SCHWAN H P. Determination of acoustic properties of blood and its components [J]. J Acoust Soc Am, 1953, 25(2): 286-289.

CHAMMAS P, EISENBERG S R. A microcontinuum model of electrokinetic coupling in the extracellular matrix: perturbation formulation and solution [J]. Journal of Colloid and Interface Science, 1994, 168(2): 526-538.

DUKHIN A S, GOETZ P J. Ultrasound for characterizing colloids [M]. Amsterdam, The Netherlands: Elsevier, 2002:21-31.

BRETSCHNEIDER F, WEILLE R J. 电生理学方法与仪器入门[M]. 封洲燕,译.北京:机械工业出版社, 2008:102-103.

DUKHIN A S, GOETZ P J. Acoustic spectroscopy for concentrated polydisperse colloids with high density contrast [J]. Langmuir, 1996, 12(21): 4987-4997.

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