The application of plasma-activated water for inactivating <italic style="font-style: italic">Malassezia furfur</italic>

HAO Xin; LIU Hongxia; CHAI Shouning

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The application of plasma-activated water for inactivating Malassezia furfur

更新时间：2025-11-18

- The application of plasma-activated water for inactivating Malassezia furfur
- Moren Journal (2025)
- 作者机构：
  
  西安交通大学能源与动力工程学院环境科学与工程系,陕西省西安市710049
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q89
- Received：09 May 2025，
  
  Revised：2025-07-21，
  
  Accepted：29 July 2025，
- 稿件说明：
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HAO Xin, LIU Hongxia, CHAI Shouning. The application of plasma-activated water for inactivating Malassezia furfur[J/OL]. Moren Journal, 2025.
DOI：

HAO Xin, LIU Hongxia, CHAI Shouning. The application of plasma-activated water for inactivating Malassezia furfur[J/OL]. Moren Journal, 2025. DOI：

摘要

针对现有灭活糠秕马拉色菌方法普适性较差，通常存在依从性低、不良反应及容易产生耐药性等问题，探索了等离子体活化水在糠秕马拉色菌灭活中的应用。通过低温等离子体技术制取不同类型的等离子体活化水，探讨物化性质的变化规律；根据菌落计数法分析糠秕马拉色菌菌体活性在灭活前后的变化规律；利用检测DNA及蛋白质泄漏量、胞外碱性磷酸酶（AKP）浓度以及紫外吸收物质含量等来分析灭活的作用机制。结果表明：在等离子体活化水中存在大量的活性物种，包括H

、H

、NO

等活性物种；当气体流量为5 L‧min

-1

和活化时间为25 min时，能够对糠秕马拉色菌造成明显的灭活效果，菌落数可降至0.98 lg（CFU‧mL

-1

）；导致菌体DNA和蛋白质泄漏、胞外AKP以及紫外吸收物质含量增加。研究可为等离子体活化水在防治糠秕马拉色菌中的应用提供理论支撑，有望成为微生物污染防治领域中绿色高效的应用方法。

Abstract

The investigation was motivated by the recognition of the limited applicability of existing methods for inactivating

Malassezia furfur

which often suffer from low compliance

adverse reactions

and the potential for drug resistance. The application of plasma-activated water in the inactivation of

Malassezia furfur

is explored. Different types of plasma-activated water are produced by low-temperature plasma technology to explore the change rule of physical and chemical properties

and the change rule of bacterial activity before and after inactivation is analyzed according to the colony counting method

DNA and protein leakage

extracellular alkaline phosphatase (AKP) concentration

and ultraviolet absorbing substance content are measured to analyze the mechanism of inactivation. The results indicate that there are a large number of active species present in plasma-activated water

including H

⁻

and other active species; When the gas flow rate is 5 L‧min

-1

and the activation time is 25 min

it can achieve a signi

ficant inactivation effect on

Malassezia furfur

reducing the colony count to 0.98 lg (CFU‧mL

-1

); This leads to increased leakage of bacterial DNA and proteins

extracellular AKP

and UV-absorbing substances. The study provides theoretical support for the application of plasma-activated water in the prevention and control of

Malassezia furfur

which is expected to become a green and efficient application method in the field of microbial pollution prevention and control.

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references

RAMÍREZ A M C , AMÉZQUITA A , JARAMILLO J E C C , et al . Analysis of Malassezia lipidome disclosed differences among the species and reveals presence of unusual yeast lipids [J ] . Frontiers in Cellular and Infection Microbiology , 2020 , 10 : 338 .

GOH J P Z , IANIRI G , HEITMAN J , et al . Expression of a Malassezia codon optimized mCherry fluorescent protein in a bicistronic vector [J ] . Frontiers in Cellular and Infection Microbiology , 2020 , 10 : 367 .

ANGIOLELLA L , CARRADORI S , MACCALLINI C , et al . Targeting Malassezia species for novel synthetic and natural antidandruff agents [J ] . Current Medicinal Chemistry , 2017 , 24 ( 22 ): 2392 – 2412 .

孙丹 , 傅宏阳 , 杨晓红 , 等 . 紫草素对糠秕马拉色菌作用下HaCaT细胞增殖的影响及其对缺氧诱导因子1α表达的调控 [J ] . 临床皮肤科杂志 , 2022 , 51 ( 5 ): 271 – 275 .

LAOKOR N , JUNTACHAI W . Exploring the antifungal activity and mechanism of action of Zingiberaceae rhizome extracts against Malassezia furfur [J ] . Journal of Ethnopharmacology , 2021 , 279 : 114354 .

程露 . 分离自不同疾病的同种马拉色菌对抗真菌药物药敏结果比较研究 [D ] . 昆明医科大学， 2016 .

RAO K J , PARIA S . Anti- Malassezia furfur activity of natural surfactant mediated in situ silver nanoparticles for a better antidandruff shampoo formulation [J ] . RSC Advances , 2016 , 6 ( 13 ): 1 1064– 11069 .

许子牧 . 大气压低温等离子体失活金黄色葡萄球菌及其机理研究 [D ] . 中国科学技术大学 , 2015 .

MA R , WANG G , TIAN Y , et al . Non-thermal plasma-activated water inactivation of food-borne pathogen on fresh produce [J ] . Journal of hazardous materials , 2015 , 300 : 643 – 651 .

SHEN J , SUN Q , ZHANG Z , et al . Characteristics of DC gas-liquid phase atmospheric-pressure plasma and bacteria inactivation mechanism [J ] . Plasma Processes and Polymers , 2015 , 12 ( 03 ): 252 – 259 .

RICHMONDS C , WITZKE M , BARTLING B , et al . Electron-transfer reactions at the plasma-liquid interface [J ] . Journal of the American Chemical Society , 2011 , 133 ( 44 ): 17582 .

XU Y , TIAN Y , MA R , et al . Effect of plasma activated water on the postharvest quality of button mushrooms, Agaricus bisporus [J ] . Food chemistry , 2016 , 197(PT.A): 436– 444 .

ZHOU R , ZHOU R , PRASAD K , et al . Cold atmospheric plasma activated water as a prospective disinfectant: the crucial role of peroxynitrite [J ] . Green Chemistry , 2018 , 20 ( 23 ): 5276 – 5284 .

覃思 , 王闰宁 , 李虎 , 等 . 等离子体射流和等离子体活化水对变形链球菌灭菌效果的研究 [J ] . 生物医学工程学杂志 , 2023 , 40 ( 3 ): 559 – 565 .

XIANG Q S , KANG C D , ZHAO D B , et al . Influence of organic matters on the inactivation efficacy of plasma-activated water against E-coli O157:H7 and S-aureus [J ] . Food Control , 2019 , 99 : 28 – 33 .

XIANG Q S , KANG C D , NIU L Y , et al . Antibacterial activity and a membrane damage mechanism of plasma-activated water against Pseudomonas deceptionensis CM2 [J ] . LWT-Food Science and Technology , 2018 , 96 : 395 – 401 .

CHEN T P , SU T L , LIANG J F . Plasma-activated solutions for bacteria and biofilm inactivation [J ] . Current Bioactive Compounds , 2017 , 13 ( 1 ): 59 – 65 .

LU X , KEIDAR M , LAROUSSI M , et al . Transcutaneous plasma stress: From soft-matter models to living tissues [J ] . Materials Science and Engineering: R: Reports , 2019 , 138 : 36 – 59 .

食品安全国家标准食品中过氧化氢残留量的测定： GB 5009226–2016 [S ] .

水质亚硝酸盐氮的测定分光光度法： GB 7492–1987 [S ] .

水质硝酸盐氮的测定紫外分光光度法（试行）： HJ/T 346–2007 [S ] .

LUKES P , DOLEZALOVA E , SISROVA I , et al . Aqueous-phase chemistry and bactericidal effects from an air discharge plasma in contact with water: evidence for the formation of peroxynitrite through a pseudo-second-order post-discharge reaction of H 2 O 2 and HNO 2 [J ] . Plasma Sources Science and Technology , 2014 , 23 ( 1 ): 015019 .

JUDÉE F , SIMON S , BAILLY C , et al . Plasma-activation of tap water using DBD for agronomy applications: Identification and quantification of long lifetime chemical species and production/consumption mechanisms [J ] . Water Research , 2018 , 133 : 47 – 59 .

RATHORE V , PATEL D , BUTANI S , et al . Investigation of physicochemical properties of plasma activated water and its bactericidal efficacy [J ] . Plasma Chemistry and Plasma Processing , 2021 , 41 ( 3 ): 871 – 902 .

盖智星 , 王日葵 , 贺明阳 , 等 . 肉桂酸钾对柑橘采后主要病原真菌的抑制作用 [J ] . 食品与发酵工业 , 2016 , 42 ( 03 ): 109 – 113 .

LV F , LIANG H , YUAN Q , et al . In vitro antimicrobial effects and mechanism of action of selected plant essential oil combinations against four food-related microorganisms [J ] . Food research international , 2011 , 44 ( 09 ): 3057 – 3064 .

HAJDU T , SZABÓ K , JAKAB A , et al . Biophysical experiments reveal a protective role of protein phosphatase Z1 against oxidative damage of the cell membrane in Candida albicans [J ] . Free Radical Biology & Medicine , 2021 , 176 : 222 – 227 .

董洁洁 . 凤仙花中1,4–萘醌类成分分离纯化及其抑菌机理的初步研究 [D ] . 北京中医药大学 , 2019 .

SUN W T , LI G , LI H P , et al . Characteristics of atmospheric-pressure, radio-frequency glow discharges operated with argon added ethanol [J ] . Journal of Applied Physics , 2007 , 101 ( 12 ): 123302 .

MCPHERSON L L . Understanding ORP’s role in the disinfection process [J ] . Water-Engineering & Management , 1993 , 140 : 29 – 31 .

TIMOSHKIN I V , MACLEAN M , WILSON M P , et al . Bactericidal effect of corona discharges in atmospheric air [J ] . IEEE Transactions on Plasma Science , 2012 , 40 ( 10 ): 2322 – 2333 .

LI J , SAKAI N , WATANABE M , et al . Study on plasma agent effect of a direct-current atmospheric pressure oxygen-plasma jet on inactivation of E. coli using bacterial mutants [J ] . IEEE Transactions on Plasma Science , 2013 , 41 ( 4 ): 935 – 941 .

LAI W S , LAI H R , KUO S P , et al . Decontamination of biological warfare agents by a microwave plasma torch [J ] . Physics of Plasmas , 2005 , 12 ( 2 ): 023501 .

ZHANG Q , LIANG Y D , FENG H Q , et al . A study of oxidative stress induced by non-thermal plasma-activated water for bacterial damage [J ] . Applied Physics Letter , 2013 , 102 : 203701 .

SUROWSKY B , FROHLING A , GOTTSCHALK N , et al . Impact of cold plasma on Citrobacter freundii in apple juice: Inactivation kinetics and mechanisms [J ] . International Journal of Food Microbiology , 2014 , 174 : 63 – 71 .

ZHANG Z L , SHEN J , CHENG C , et al . Generation of reactive species in atmospheric pressure dielectric barrier discharge with liquid water [J ] . Plasma Science and Technology , 2018 , 20 ( 4 ): 61 – 66 .

QI Z H , TIAN E Q , SONG Y , et al . Inactivation of Shewanella putrefaciens by plasma activated water [J ] . Plasma Chemistry and Plasma Processing , 2018 , 38 ( 5 ): 1035 – 1050 .

陈玥 . 低温等离子体对生鲜湿面表面杀菌研究及装置开发 [D ] . 天津科技大学 , 2017 .

ARANA I , SANTORUM P , MUELA A , et al . Chlorination and ozonation of waste‐water: comparative analysis of efficacy through the effect on Escherichia coli membranes [J ] . Journal of applied microbiology , 1999 , 86 ( 5 ): 883 – 888 .

朱育攀 . 大气压低温等离子体对金黄色葡萄球菌的杀菌效应研究 [D ] . 郑州大学 , 2019 .

BHUNIA A . Microbial foodborne pathogens: mechanisms and pathogenesis, second edition, springer-nature [J ] . Microbial Foodborne Pathogens: Mechanisms and Pathogenesis , Second Edition , Springer-Nature, 2018 .

KANEKO T , SASAKI S , TAKASHIMA K , et al . Gas-liquid interfacial plasmas producing reactive species for cell membrane permeabilization [J ] . Journal of Clinical Biochemistry and Nutrition , 2017 , 60 ( 1 ): 3 – 11 .

白妍 . 等离子体活化水对蜡样芽孢杆菌（ B .cereus）芽孢的杀灭效果与机理研究[D ] . 浙江大学 , 2020 .

沈超怡 , 吴清燕 , 饶景珊 , 等 . 等离子水处理对马铃薯杀菌保鲜效果的影响 [J ] . 包装工程 , 2019 , 40 ( 19 ): 9 – 15 .

CHEN D , CHEN P , CHENG Y , et al . Deoxynivalenol decontamination in raw and germinating barley treated by plasma-activated water and intense pulsed light [J ] . Food and Bioprocess Technology , 2019 , 12 ( 02 ): 246 – 254 .

XIANG Q , LIU X , LIU S , et al . Effect of plasma-activated water on microbial quality and physicochemical characteristics of mung bean sprouts [J ] . Innovative Food Science & Emerging Technologies , 2019 , 52 , 49 – 56 .

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⁰

The application of plasma-activated water for inactivating Malassezia furfur

DOI：

摘要

Abstract

关键词

Keywords

references