In Situ Remediation of Nitrobenzene-Contaminated Groundwater Using Emulsified Zero-Valent Iron Reduction Coupled with Microbial Oxidation

XU Baiqing; ZHAO Yue; CHEN Mingxiang; SHAN Guangbo; Li Aimin; ZHANG Lei

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In Situ Remediation of Nitrobenzene-Contaminated Groundwater Using Emulsified Zero-Valent Iron Reduction Coupled with Microbial Oxidation

更新时间：2025-08-21

- In Situ Remediation of Nitrobenzene-Contaminated Groundwater Using Emulsified Zero-Valent Iron Reduction Coupled with Microbial Oxidation
- Moren Journal (2025)
- 作者机构：
  
  1.大连理工大学环境学院,辽宁省大连市116024
  2.中石化（大连）石油化工研究院有限公司,辽宁省大连市116045,中国
- 作者简介：
- 基金信息：
- DOI：
  CLC： X523
- Received：29 April 2025，
  
  Revised：2025-06-27，
  
  Accepted：02 July 2025，
- 稿件说明：
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XU Baiqing, ZHAO Yue, CHEN Mingxiang, et al. In Situ Remediation of Nitrobenzene-Contaminated Groundwater Using Emulsified Zero-Valent Iron Reduction Coupled with Microbial Oxidation[J/OL]. Moren Journal, 2025.
DOI：

XU Baiqing, ZHAO Yue, CHEN Mingxiang, et al. In Situ Remediation of Nitrobenzene-Contaminated Groundwater Using Emulsified Zero-Valent Iron Reduction Coupled with Microbial Oxidation[J/OL]. Moren Journal, 2025. DOI：

摘要

为破解在产企业场地污染“边生产、边修复”难题，针对乳化零价铁还原耦合微生物氧化技术在硝基苯污染地下水中的应用开展了实验研究。首先，构建了室内砂箱反应系统，系统考察了乳化零价铁还原硝基苯效率的影响因素，评价了苯胺的生物降解规律及关键功能菌群。接着，在污染场地开展了化学还原-生物氧化反应带中试，考察了水质参数动态变化及污染去除效果。砂箱实验表明，酸性条件（pH = 4）显著提升还原效率，5 h硝基苯去除率达99.82%；小粒径乳化零价铁更具活性，经济最优铁/硝基苯质量比为1.2 ~ 1.6。苯胺降解菌群呈现浓度依赖性，浓度低于200 mg/L时降解率可达95%以上，1200 mg/L时受抑制降至52.13% ~ 62.21%，Pseudomonadota和Planctomycetota为优势功能菌属。现场中试结果表明，乳化零价铁还原-微生物氧化串联修复技术可有效去除地下水中的硝基苯和苯胺，上游化学反应带30天内硝基苯去除率达95.69%，下游生物反应带对累积苯胺的降解率为89.06%。该研究可为在产企业污染地下水原位绿色修复提供数据参考。

Abstract

To address the challenge of implementing "remediation while production continues" at operational industrial sites

experimental research was conducted on the application of emulsified zero-valent iron reduction coupled with microbial oxidation for treating nitrobenzene-contaminated groundwater. First

a laboratory-scale sandbox reaction system was constructed to systematically investigate factors influencing nitrobenzene reduction efficiency by emulsified zero-valent iron and evaluate the biodegradation patterns of aniline and key functional microbial consortia. Subsequently

a pilot-scale in-situ chemical reduction-biological oxidation reaction zone was established at a contaminated site to examine dynamic variations in water quality parameters and contaminant removal efficacy. Sandbox experiments demonstrated that acidic conditions (pH = 4) significantly enhanced reduction efficiency

achieving 99.82% nitrobenzene removal within 5 hours. Smaller emulsified zero-valent iron particles exhibited higher reactivity

with an economically optimal iron/nitrobenzene mass ratio of 1.2 ~ 1.6. The

aniline-degrading microbial consortium displayed concentration-dependent activity: degradation rates exceeded 95% at concentrations below 200 mg/L but declined to 52.13% ~ 62.21% at 1

200 mg/L due to inhibition.

Pseudomonadota

and

Planctomycetota

were identified as dominant functional genera. Pilot-scale results confirmed the effectiveness of the emulsified zero-valent iron reduction-microbial oxidation sequential technology

with the upstream chemical reduction zone achieving 95.69% nitrobenzene removal within 30 days

while the downstream biological oxidation zone degraded 89.06% of accumulated aniline. This study provides critical data support for in-situ green remediation of contaminated groundwater at operational industrial facilities.

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references

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